| 一种新的斑马鱼cloche突变体亚型的基因鉴定 |
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| 引用本文： | 马宁,霍中军,颜广,黄红辉,罗深秋,张文清.一种新的斑马鱼cloche突变体亚型的基因鉴定[J].南方医科大学学报,2010,30(3):458. |
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| 作者姓名： | 马宁 霍中军 颜广 黄红辉 罗深秋 张文清 |
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| 作者单位： | 1. 南方医科大学细胞生物学教研室,广东,广州,510515
2. 南方医科大学遗传学教研室,广东,广州,510515 |
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| 基金项目： | 国家自然科学基金(30828020,30671074);;广东省自然科学基金(7005144,06024376);;广东省教育厅课题(05Z006) |
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| 摘 要： | 目的斑马鱼cloche172突变体的基因鉴定。方法采用化学诱变剂N-乙基-N-亚硝基脲(N-ethyl-N-nitrosourea,ENU)诱变雄性野生型AB斑马鱼,实施大规模正向遗传学方法筛选髓系细胞标记物lysozymeC(lyC)表达缺失突变体,并对其中一个名为cloche172突变体进行大体形态学观察,基因定位克隆和遗传学互补实验。结果大规模正向遗传学筛选出4个lyc表达缺失突变体,其中一个cloche172突变体受精后3d(day post fertilization,dpf)时期大体形态改变与已知但基因不明的cloche突变体相似。遗传学互补实验观察到有1/4胚胎出现类似cloche突变体的表型。同时,cloche172突变体基因定位在13号染色体近端粒的区域,与cloche突变体基因定位区域一致。而o-dianisdine实验证实cloche172突变体有较多红细胞聚集,cloche突变体仅有少量在尾部存在。结论cloche172基因与cloche基因定位一致,cloche172突变体是一个新的点突变位点的cloche突变体。
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| 关 键 词： | 斑马鱼ENU诱变 大规模lyC缺失突变体筛选 cloche172突变体 造血过程 |
Positional cloning of a novel allele of zebrafish cloche mutant |
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| MA Ning,HUO Zhong-jun,YAN Guang,HUANG Hong-hui,LUO Shen-qiu,ZHANG Wen-qing.Positional cloning of a novel allele of zebrafish cloche mutant[J].Journal of Southern Medical University,2010,30(3):458. |
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| Authors: | MA Ning HUO Zhong-jun YAN Guang HUANG Hong-hui LUO Shen-qiu ZHANG Wen-qing |
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| Institution: | MA Ning1,HUO Zhong-jun1,YAN Guang1,HUANG Hong-hui2,LUO Shen-qiu1,ZHANG Wen-qing1 1Department of Cell Biology,2Department of Genetics,Southern Medical University,Guangzhou 510515,China |
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| Abstract: | Objective To perform the genetic idencification of cloche172 mutant zebrafish. Methods The chemical mutagen N-ethyl-N-nitrosourea (ENU) was used to treat the AB stain male fish. Large-scale forward genetic screening was carried out to search for lyC-deficient zebrafish mutant by WISH. The morphology changes of the embryos at 3 days postfertilization (3dpf) stage were observed and the cloche172 gene was identified by mapping and complementation test. Results We selected 4 lyC-deficient zebrafish by WISH. clo... |
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| Keywords: | N-ethyl-N-nitrosourea mutagenesis Zebrafish large-scale lyC-deficient mutant screening cloche172 mutant hematopoiesis |
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