Effect of estradiol on heme biosynthetic pathway in lead-poisoned rabbits |
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Authors: | Shoko Ohmori Koichi Harada Chang Nian Wei Qingjum Wei Atsushi Ueda |
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Institution: | (1) Department of Preventive and Environmental Medicine, Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, 1-1-1, Honjo, 860-8556 Kumamoto, Japan;(2) Department of Microbiology and Environmental Chemistry School of Health Science, Kumamoto University, Kumamoto, Japan;(3) Center for Policy Studies, Kumamoto University, Kumamoto, Japan |
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Abstract: | Objectives To clarify the effect of the female hormone estradiol (Est) on heme biosynthesis in lead-poisoned rabbits, parameters indicating
lead exposure, such as free erythrocyte protoporphyrin (FEP) level and δ-aminolevulinic acid dehydratase (ALA-D) activity,
were determined.
Methods Twenty-six male Japanese white rabbits (body weight (BW), 3kg) were divided into four groups: I (control), II (Est), III (Pb),
IV (Est+Pb). About 3 weeks after castration, Est (3 mg/kg of BW) was injected intramuscularly, and 2 weeks thereafter, lead
(1.2 mg/kg of BW) was injected intravenously. After the initial injection of each of these substances, the same dose of each
of these substances was injected once a week until the 9th week.
Results In groups III and IV, FEP level increased and ALA-D activity in the erythrocytes, bone marrow and liver decreased with an
increase in lead concentration in blood. FEP level decreased significantly (p<0.01) in the 8th and 10th weeks after Est injection
in group IV compared to with that in group III and was not elevated in group II compared with that in group I. ALA-D activity
in the erythrocytes, bone marrow and liver increased significantly in group II compared with that in group I, whereas Ht and
Hb levels decreased in group II compared with those in group I, and decreased in group IV compared with those in group III.
The level of iron in plasma (Fe−P) was within the normal range during experiment.
Conclusions In this study, Est did not increase FEP level. From the above results regarding FEP level and ALA-D activity, Est may prevent
an increase in FEP level caused by lead. Ht and Hb levels, which are the parameters of anemia, decreased mainly as a result
of Est exposure rather than lead exposure. |
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Keywords: | estradiol lead poisoning free erythrocyte protoporphyrin (FEP) δ -aminolevulinic acid dehydratase (ALA-D) activity rabbit |
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