中药"拮新康"对白血病细胞凋亡的影响

为探讨中药“拮新康”对白血病细胞凋亡的影响。采用形态学及DNA凝胶电泳方法观察其对白血病细胞株HL 6 0及K5 6 2细胞凋亡的影响 ,结果示 :①电镜观察可见凋亡细胞和凋亡小体 ,DNA凝胶电泳可见典型的梯形带改变 ;②一定浓度范围内的拮新康经适当作用时间后可诱导白血病HL 6 0 ,K5 6 2细胞凋亡 ,但诱导K5 6 2细胞凋亡所需时间较HL 6 0长且剂量较大。提示拮新康可诱导白血病细胞凋亡 ,与剂量和时间有一定的关系 ,为临床治疗白血病提供了实验依据。

The effects of jiexinkang on the apoptosis of leukemic cells

The effects of Chinese medicine jiexinkang(JXK) on apoptosis of leukemic cells were studied by morphology approach and electrophoresis of DNA fragments. Results: ①The apoptotic cells and apoptotic bodies were found by electrical microscopy and the typical ladders of DNA fragments were detected by electrophoresis. ②JXK induced apoptosis of leukemic cells(HL 60 and K562) in a certain range of concentration and at appropriate time. The time to K562 cell apoptosis was longer than that of HL 60 and its dosage was larger than that of HL 60. Conclusion: HL 60 and K562 leukemic cell apoptosis may be induced by JXK and there is correlation between dose and time. The study provides experimental evidences for the clinical treatment of leukemia.