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A method for the organ explant culture of fish kidney tubules
Authors:Raymond T. Jones  James E. Klaunig  Hayato Sanefuji  David E. Hinton
Affiliation:(1) Department of Pathology Comparative and Environmental Pathobiology Program, University of Maryland School of Medicine, 21201 Baltimore, Maryland, USA;(2) Present address: Department of Pathology, Medical College of Ohio, 43699 Toledo, OH, USA;(3) Present address: Department of Pathology, University of Occupational and Environmental Health, School of Medicine, 807 Yahatanish-ku, Kitakyushu-city, Japan;(4) Present address: Department of Anatomy, University of West Virginia Medical Center, 26506 Morgantown, WV, USA
Abstract:Summary A method for long-term organ explant culture of isolated fish kidney tubules from two teleost species is provided. Tubules maintained normal structures for 8 weeks when cultured in supplemented CMRL-1066 medium in controlled atmosphere chambers.
Keywords:fish nephron culture  teleost  catfish  hogchoker
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