CA125、Cyt-c和MGMT基因在卵巢癌患者血清中的表达及临床意义

目的:探讨糖类抗原125(carbohydrate antigen 125,CA125)联合细胞色素C(Cytochromes c,Cyt-c)和O6-甲基鸟嘌呤-DNA-甲基转移酶(MGMT)在卵巢癌患者血清中的表达情况及临床意义.方法:对53例卵巢癌患者(卵巢癌组)、40例卵巢良性病变患者(卵巢良性病变组)、50例同期体检健康女性(对照组)采用电化学发光法检测血清CA125水平,ELISA法检测Cyt-c水平,甲基化特异PCR法检测MGMT基因启动子甲基化,流式细胞仪检测细胞线粒体膜势能.结果:卵巢癌患者血清中CA125和Cyt-c表达水平明显高于卵巢良性肿瘤组和健康对照组(P<0.05),CA125表达水平在卵巢良性肿瘤组和健康对照组,差异无统计学意义(P>0.05),Cyt-c差异有统计学意义(P<0.05).MGMT基因启动子在卵巢癌组和卵巢良性肿瘤组中发生甲基化,甲基化率分别为75.3%和21.8%,健康对照组未发生甲基化.结论:联合检测血清CA125、Cyt-c和MGMT基因的表达水平有助于提高早期卵巢癌的诊断效能,对卵巢肿瘤的良恶性鉴别及对预后具有提示作用.

The expression and clinical value of serumm carbonhydrate antigen 125 ,Cytochromes c and MGMT in patients with ovarian cancer

Objective:To investigate the expression of CA125,Cyt-c and O6 -methylguanine DNA methyltrans-ferase( MGMT)and its clinical significance in cervical cancer. Methods:Methylation-specific PCR was used to de-tect the MGMT methylation status,mitochondrial Transmembrane potentials were examined using flow cytometric anal-ysis. The serum level of CA125 was detected by electro-chemiluminescence,the level of Cyt-c was detected by ELISA respectively in 53 patients with ovarian cancer( cancer group),40 patients with benign ovarian tumor( benign group)and 50 healthy females( control group). Results:The level of CA125 and MGMT methylation status was sig-nificantly higher in cancer group than those in benign group and control group,the positive rates were 75. 3% and 21. 8% respectively(P<0. 05). Cyt-c was lower in cancer group than those in benign group and control group. Conclusion:The combined detection of CA125,Cyt-c and MGMT methylation status contributes to improving the di-agnosis efficiency,and has a certain application value to the differential diagnosis of benign and malignant ovarian tumor.