| 白杨素诱导白血病细胞HL-60凋亡及其机制 |
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| 引用本文： | 张嵘,王洪涛,苗苗,李佳,杨莹,刘卓刚.白杨素诱导白血病细胞HL-60凋亡及其机制[J].现代肿瘤医学,2016,0(10):1522-1524. |
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| 作者姓名： | 张嵘 王洪涛 苗苗 李佳 杨莹 刘卓刚 |
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| 作者单位： | 中国医科大学附属盛京医院血液内科，辽宁 沈阳 110004 |
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| 摘 要： | 目的:检测白杨素诱导白血病细胞HL-60凋亡及其机制。方法:分别利用MTT实验和Annexin V-FITC/PI双染实验检测细胞增殖和凋亡。Western blot技术检测相关通路蛋白变化。结果:白杨素可以明显诱导白血病细胞HL-60凋亡。白杨素处理后细胞的p-AKT蛋白水平明显下降。结论:白杨素能够通过AKT通路诱导HL-60细胞凋亡。
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| 关 键 词： | HL-60细胞 白杨素 增殖 凋亡 AKT |
Chrysin induces apoptosis in HL - 60 cells and its mechanisms |
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| Zhang Rong,Wang Hongtao,Miao Miao,Li Jia,Yang Ying,Liu Zhuogang.Chrysin induces apoptosis in HL - 60 cells and its mechanisms[J].Journal of Modern Oncology,2016,0(10):1522-1524. |
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| Authors: | Zhang Rong Wang Hongtao Miao Miao Li Jia Yang Ying Liu Zhuogang |
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| Institution: | Hematology Department In Shengjing Hospital of China Medical University,Liaoning Shenyang 110004,China. |
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| Abstract: | Objective:To detect the effects and mechanisms of Chrysin on human leukemia cell HL - 60. Meth-ods:The proliferation and apoptosis of HL - 60 cells after treated with Chrysin were detected by using MTT assay and Annexin V - FITC/ PI double staining,respectively. Western blot was used to detect the mechanism of Chrysin. Re-sults:Chrysin could induce the apoptosis in HL - 60 cells. The level of p - AKT was lower in treated cells than un-treated ones. Conclusion:Chrysin could induce apoptosis in HL - 60 cells by suppressing p - AKT. |
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| Keywords: | HL-60 cells Chrysin proliferation apoptosis AKT |
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