PAGE5对非小细胞肺癌细胞生长、凋亡和侵袭能力的影响

目的：探讨前列腺相关基因5（PAGE5）在非小细胞肺癌（NSCLC）中的表达及其对肺癌细胞生物学行为的影响。方法：采用实时定量PCR和Western blot方法检测37例NSCLC及其癌旁组织中PAGE5的表达；采用MTT法、流式细胞术、Transwell以及Western blot法检测转染PAGE5 siRNA对A549和H1299细胞生长、凋亡、侵袭以及Bax、Bcl－2和MMP－2表达的影响。结果：在37．84％（14／37）的肺癌组织样本中PAGE5基因表达上调。转染PAGE5 siRNA的A549和H1299细胞生长无明显变化、细胞凋亡显著增加、侵袭能力显著下降（P＜0．05），Bax蛋白表达显著上调，Bcl－2和MMP－2蛋白表达显著下调（P＜0．05）。结论：PAGE5可能通过调控Bax、Bcl－2及MMP－2蛋白的表达影响NSCLC细胞的生长、凋亡与侵袭，有望成为肺癌诊断标志物及潜在的治疗靶点。

The effect of PAGE5 expression on growth,apoptosis and invasion of non -small cell lung cancer cells

Objective:To investigate the expression of prostate-associated gene 5 (PAGE5 )in non-small cell lung cancer(NSCLC)and its biological effect in NSCLC cells.Methods:Quantitative Real -time RT-PCR and Western blot were performed to detect mRNA and protein level of PAGE5 in 37 cases of NSCLC compared with adja-cent non-cancerous lung tissues.MTT assay,flow cytometry,Transwell assay and Western blot were used to evaluate cell growth,apoptosis,invasion and protein level of Bax,Bcl-2 and MMP-2 after transfection of PAGE5 siRNA in A549 and H1299 cells.Results:The expression of PAGE5 in HCC samples was significantly higher than 37.84%(14/37)in adjacent normal lung tissues.Silencing of PAGE5 in A549 and H1299 cells slightly inhibited cell growth, significantly promoted cell apoptosis,depressed cell invasion(P<0.05 ),increased Bax and decreased Bcl-2 and MMP-2 protein expression(P<0.05).Conclusion:PAGE5 may act as a positive regulator to NSCLC by regulation of Bax,Bcl-2 and MMP-2 protein expression and may be used as a new marker or a potential therapeutic target for NSCLC.