| CNTN1基因对乳腺癌细胞株Hs578T增殖、迁移及侵袭能力的影响 |
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| 引用本文： | 陈楠,贺赛,丁艳妮,宋张骏,韩丕华,杨晓民,王虎霞,刘华,袁权.CNTN1基因对乳腺癌细胞株Hs578T增殖、迁移及侵袭能力的影响[J].现代肿瘤医学,2016(19):3017-3020. |
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| 作者姓名： | 陈楠 贺赛 丁艳妮 宋张骏 韩丕华 杨晓民 王虎霞 刘华 袁权 |
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| 作者单位： | 陕西省肿瘤医院,陕西 西安,710061 |
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| 基金项目： | 陕西省卫生厅科研基金扶植项目(编号2014D14) |
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| 摘 要： | 目的：研究CNTN1基因对乳腺癌细胞株Hs578T细胞增殖、克隆形成、迁移及侵袭能力的影响。方法：将前期构建的稳定表达pEGFP－N1－CNTN1的Hs578T细胞系，采用MTT、流式细胞仪技术、平板克隆实验及Transwell实验检测pEGFP－N1－CNTN1对Hs578T细胞增殖、克隆形成、迁移及侵袭能力的影响。结果：与空白组及对照组相比，过表达CNTN1基因的Hs578T细胞增殖能力、克隆形成、迁移及侵袭能力增强（P＜0．05）。结论：CNTN1基因能够增强Hs578T细胞的增殖、克隆形成、迁移及侵袭能力。
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| 关 键 词： | 乳腺癌 CNTN1 增殖 克隆形成 迁移 侵袭 |
Effects of recombinant plasmid of pEGFP -N1 -CNTN1 on proliferation,clone formation,migration and invasion of Hs578T cells |
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| Abstract: | Objective:To explore the effects of pEGFP-N1 -CNTN1 on proliferation,clone formation and migra-tion of Hs578T cells.Methods:The effects of proliferation,clone formation and migration invasion of Hs578T cells were examined by MTT,clone formation assay and Transwell assay.Results:Hs578T cells transfected with pEGFP-N1 -CNTN1 plasmid expressed a higher level of CNTN1 protein steadily.The capabilities of proliferation,clone for-mation and migration of Hs578T cells increased significantly compared with blank control group(P<0.05).Conclu-sion:CNTN1 gene can enhance the abilities of proliferation,clone formation,migration and invasion of Hs578T cells. |
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| Keywords: | breast cancer CNTN1 proliferation clone formation migration invasion |
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