PEG －rhG －CSF 预处理对 BUCY 方案致小鼠骨髓抑制模型骨髓造血恢复的影响

目的：研究聚乙二醇化粒细胞集落刺激因子（PEG －rhG －CSF）预处理后不同时间间隔对 BUCY 方案致小鼠骨髓抑制模型骨髓及外周血象恢复情况的影响,为进一步扩展 PEG －rhG －CSF 的临床应用范围提供理论依据。方法：BLAB／c 雌性小鼠50只,随机分为5组（n ＝10）。实验组（Group6、Group10、Group13、Group16）皮下注射 PEG －rhG －CSF 70mg·kg －1,给药后的第6、10、13及16天给予白消安（BU）35mg·kg －1· d －1连续4天,之后给予环磷酰胺（CY）50mg·kg －1·d －1连续2天。粒细胞缺乏组（Model）给予 BU 35mg· kg －1·d －1连续4天,之后给予 CY 50mg·kg －1·d －1连续2天。结果：经 BUCY 方案处理后,除 Group6组白细胞于第5天开始下降外,其余各组白细胞均于第3天开始下降;各组的（Model、Group6、Group10、Group13、Group16）白细胞最低值分别出现在第11、9、9、11及11天,白细胞恢复正常的时间分别为第19、23、23、21及19天。第19天时 Model 和 Group16组的骨髓病理结果均已趋于正常,其他组骨髓组织还存在不同程度的空泡结构,表现为低增生骨髓状态。结论：使用 PEG －rhG －CSF 后16天对于 BUCY 方案引起的小鼠骨髓抑制模型的骨髓恢复已无影响,临床5天用药的3周化疗方案可以尝试性使用 PEG －rhG －CSF 预防粒细胞降低。

The effect of pretreated PEG -rhG -CSF on the hematopoietic recovery of bone marrow of the myelosuppressed mice caused by BUCY

Objective:To research the effect of bone marrow and peripheral blood of myelosuppresived mice trea-ted by busulfan and cyclophosphamide(BUCY)applied by PEG -rhG -CSF in different time intervals.Methods:BLAB/c female mice were randomly divided into 5 groups(n =10),the mice in the test groups(Group6 ,Group10 , Group13 ,Group16 )were injected subcutaneously by PEG -rhG -CSF 70mg·kg -1 ,and then treated by BU 35mg· kg -1 ·d -1 for 4 days and CY 50mg·kg -1 ·d -1 for 2 days on the 6 th ,10th ,13 th ,16 th day.The control group(Model) were only treated by BU 35mg·kg -1 ·d -1 for 4 days and CY 50mg·kg -1 ·d -1 for 2 days.Results:After BU used, the white blood cell of all the groups began to decline from the 3 th day except Group6 was from the 5 th day.The mini-mum of white blood cell of all the groups(Model,Group6 ,Group10 ,Group13 ,Group16 )appeared on the 11 th ,9 th ,9 th , 11 th ,11 th day,and then restored to the normal level on the 19 th ,23 th ,23 th ,21 th and 19 th day.On the 19 th day after BU used,the bone marrow features were generally normal for Model and Group16 ,and the myeloproliferative were poorer for the other groups.Conclusion:There was no influence for the recovery of bone marrow of the mice,treated by BU 35mg·kg -1 ·d -1 for 4 days and CY 50mg·kg -1 ·d -1 for 2 days on the 16 th day after injected subcutaneously by PEG -rhG -CSF.So we can prevent the decline of white blood cells by PEG -rhG -CSF in the chemotherapy which treatment time last 5 days per 3 weeks for clinical application.