沉默AFAP-1L2对胃癌MKN-28细胞的生物学影响

目的:探讨AFAP-1L2对胃癌MKN-28细胞的生物学影响。方法:siRNA干扰AFAP-1L2；CCK-8检测沉默AFAP-1L2后细胞的增殖情况；免疫荧光分析各组细胞的凋亡率；Transwell小室观察细胞的侵袭情况；Real time-PCR检测细胞中基因的表达情况。结果:siRNA有效干扰了AFAP-1L2的表达；沉默AFAP-1L2细胞组的细胞增殖明显受到抑制（P<0.05）；siRNA-AFAP-1L2组细胞的凋亡率与其他组比较，凋亡率增高；细胞的侵袭情况，沉默AFAP-1L2明显抑制了细胞的侵袭，与其他两组比较差异显著（P<0.05）；ERK1/2、p-ERK1/2和Bcl-2的表达水平，均是siRNA-AFAP-1L2细胞组低于其他两组（P<0.05）。结论:沉默AFAP-1L2抑制了胃癌MKN-28细胞的增殖，诱导细胞的凋亡，抑制了细胞的侵袭和抗凋亡基因的表达，AFAP-1L2可以通过ERK1/2途径发挥作用，为肿瘤的靶向治疗提供一定的理论依据。

The biological effects on gastric MKN-28 cells by silencing AFAP-1 L2

Objective:To explore the biological effects on gastric MKN-28 cells by AFAP-1L2.Methods:siRNA interfer AFAP-1L2.Using CCK-8 to detect the proliferation of cells with AFAP-1L2 silence.To analyse the condition of cell apoptosis by immunofluroescence.Using Transwell to detect the invasion ability.The expression of genes in MKN-28 cells were analysed by Real time-PCR.Results:siRNA effectively interfered the expression of AFAP-1L2.Cell proliferation was significantly suppressed in siRNA-AFAP-1L2 cell group(P<0.05).The apoptosis rate of siRNA-AFAP-1L2 was significantly higher than other groups.The cell counts of siRNA-AFAP-1L2 group was lower than other groups(P<0.05).Real time-PCR showed that the expression of ERK1/2，p-ERK1/2 and Bcl-2 in siRNA-AFAP-1L2 group was lower than those in other groups(P<0.05).Conclusion:Silence of AFAP-1L2 inhibited gastric cancer MKN-28 cell proliferation and induced the apoptosis of cells，inhibited invasion and antiapoptotic gene expression，AFAP-1L2 may play a role by ERK1/2 signaling pathway，these results provided certain theoretical basis for targeting therapy of gastric cancer.