下调 miR -181a 对人胃癌细胞生物学行为的影响

目的：探讨下调 miR -181a 对人胃癌细胞生物学行为的影响。方法：转染 miR -181a inhibitor 下调miR -181a 在人胃癌细胞 SGC -7901中的表达。通过 MTT 法、流式细胞仪、Annexin - V + PI 双染色法和 Tr-answell 实验，观察下调 miR -181a 表达对人胃癌细胞增殖、凋亡及侵袭迁移等生物学行为的影响。结果：瞬时转染 miR -181a inhibitor 后胃癌细胞 SGC -7901中 miR -181a 的表达明显下调（P =0.0027）。转染 miR

The effect of down - regulating miR - 181a on biological behavior of gastric cancer cell

Objective:To investigate the influence of down - regulating miR - 181a on biological behavior of gas-tric cancer cell. Methods:miR - 181a inhibitor was transfected transiently into gastric cancer cell SGC - 7901 to down - regulate the expression of miR - 181a. The influence of down - regulation of miR - 181a on cell proliferation, apoptosis,invasion and migration were evaluated by MTT,flow cytometry,Annexin - V + PI and transwell assay. Results:The transfection of miR - 181a inhibitor down - regulated miR - 181a expression in SGC - 7901(P = 0. 002 7). In SGC - 7901 cell after transfection with miR - 181a inhibitor,the proliferative ability was inhibited(P = 0. 000 6), G0 / G1 phase cell ratio was arrested(P = 0. 007 5),S phase cell ratio was decreased(P = 0. 002 2),and apoptosis rate was increased(P < 0. 001 7). Transwell assay showed cell invasion and migration was increased greatly in miR -181a down - regulated SGC - 7901 cell(P = 0. 000 6,P = 0. 000 4). Conclusion:Down - regulation of miR - 181a inhibits gastric cancer cell proliferation and promotes apoptosis,but enhances cell invasion and migration. It may play an important role in gastric carcinogenesis.