深圳市学龄前儿童流感嗜血杆菌带菌情况及分型研究

目的了解深圳市健康学龄前儿童口咽部流感嗜血杆菌（Haemophilus influenza，Hi）携带情况及菌型特点。方法选择深圳市具有代表性的3所日托幼儿园健康儿童181名，进行口咽部Hi携带率监测，Hi分离株进一步作生物学分型、血清分型及PFGE分子分型。结果从181份样品中共分离到Hi45株，阳性率为24．9％。生物学分型分别为Ⅰ型2株（4．4％）、Ⅱ型13株（28．9％）、Ⅲ型18株（40．0％）、Ⅳ型2株（4．4％）、Ⅴ型2株（4．4％）、Ⅵ型2株（4．4％）、Ⅶ型0、Ⅷ型6株（13．3％）。血清分型为不可分型44株，占97．8％（44／45），b型为1株，占2．2％（1／45）。PFGE分型：39株血清不可分型}H分为38个基因型。结论深圳市学龄前健康儿童口咽部Hi携带率为24．9％，与国内南方各大城市无差异，生物学分型以Ⅱ型、Ⅲ型为主，PFGE结果显示，深圳市学龄前儿童携带的Hi菌株基因高度多态性，无优势菌株。

Study on the status of oral pharyngeal carriage and typing of Haemophilus influenza in healthy preschool in Shenzhen

Objective To study on the status of oral pharyngeal carriage and characteristics of Haemophilus influenza from healthy preschool in Shenzhen. Methods One hundred and eighty-one children in three representative kindergartens in Shenzhen were studied as research subjects, and the carriage rates of Hi were studied. All Hi strains were typinged in biology,serum and PFGE. Results 45 strains Hi were separated from 181 samples, the positive rate was 24. 9%. Biology Ⅰ -Ⅲ were 2 strain,13 strain.18 strain,2 strain.2 strain,2 strain,0 strain,6 strain. Serotyping ： NTHi were 44 strain, Hib were 1 strain; Genotyping： There were 38 Genotyping in 39 strain NTHi. Conclusion The status of oral pharyngeal carriage of Haemophilus influenza was 24.9% in healthy preschool in Shenzhen, there was no difference between Shenzhen and other cities of south China, Major of biology typing were Ⅱ and Ⅲ. As a result, Genotyping have polymorphism and no ascendancy strain.