阳春砂杂交子代与母本的FTIR鉴别研究

目的 探索阳春砂杂交子代与母本的红外光谱鉴别方法。方法 采用傅里叶变换红外光谱法（FTIR）测定阳春砂杂交子代与母本的红外光谱，分别应用导数光谱和傅里叶自解卷积对图谱进行转换处理，对比寻找杂交子代与母本的特征差异吸收。结果 阳春砂杂交子代与母本的红外光谱无明显差异，经四阶导数光谱变化后于1 051.014 cm^?1处出现明显的特征差异吸收峰，傅里叶自解卷积转化后于771.563 4和1 612.432 5 cm^?1处出现了具有明显特征独立的差异吸收峰。结论 阳春砂杂交子代与母本的FTIR经过导数光谱和傅里叶自解卷积转化后可出现明显的差异，能较好地鉴别二者。

FTIR identification between Amomum villosum hybrid offspring and female parent

Objective To study the identification between the Amomum villosum hybrid offspring and the female parent by useing the Fourier transform infrared (FTIR) spectroscopy. Methods Using the FTIR to determine the infrared spectra of A.villosum hybrid offspring and female parent. Switching and processing the atlas with derivative spectrometry and Fourier self-deconvolution, to contrast for the characteristic differences in absorbance of them. Results There were no significant differences between the A. villosum hybrid offspring and female parent by using FTIR. But 1 051.014 cm^?1 was found to be the most obvious in the fourth-derivative spectrum, 771.563 4 and 1 612.432 5 cm^?1 to be the most obvious in the Fourier self-deconvolution spectra. Conclusion There are obvious differences between the A. villosum hybrid offspring and female parent in the derivative spectrometry and Fourier self-deconvolution spectroscopy atlas. Therefore, this method can be used to identify the A.villosum hybrid offspring and female parent simply, rapidly, and accurately.