| 定量无梗五加果提取物的体内抗血栓与抗血小板凝集活性(英文) |
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| 引用本文： | 宋洋,杨春娟,于凯,李发美.定量无梗五加果提取物的体内抗血栓与抗血小板凝集活性(英文)[J].中国天然药物,2011(2):141-145. |
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| 作者姓名： | 宋洋 杨春娟 于凯 李发美 |
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| 作者单位： | 沈阳药科大学分析化学教研室; |
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| 基金项目： | supported by 2009 Key Laboratory of Colleges and Universities Plan Scientific Research Projects (No. 2009S101)~~ |
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| 摘 要： | 目的:研究无梗五加果乙醇提取物的大鼠体内抗血栓与抗血小板凝集活性及其三萜皂苷类成分。方法:采用大鼠动-静脉旁路血栓模型的测定无梗五加果醇提物的体内抗血栓活性;采用血小板凝集仪测定其体内抗ADP诱导的血小板凝集活性;采用UPLC-MS/MS法同时对无梗五加根提取物中10种三萜皂苷类成分进行含量测定并采用比色法对其总三萜皂苷成分的含量进行了测定。结果:连续灌胃给予大鼠无梗五加醇提物(125,250,500及1000mg·kg-1/d)15天后,其体内抗血栓活性显示出剂量依赖关系(P<0.05)。同时,提取物也显著抑制了大鼠体内ADP诱导的血小板凝集活性(P<0.05)。不同剂量提取物(125,250,500及1000mg/kg·d-1)下的抑制率分别为(16.3±4.7)%,(28.6±4.1)%,(47.9±4.0)%与(61.3±6.6)%,阳性对照阿司匹林组(27mg·kg-1/d)的抑制率为(55.4±7.5)%。提取物中3个主要三萜皂苷成分22α-hydroxychiisanoside,momordin Ib以及chiisanogenin的含量分别为(3755±51.3),(2461±73.0)和(11...
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| 关 键 词： | 无梗五加果 体内抗血栓活性 抗血小板凝集活性 旁路血栓模型 三萜 UPLC-MS/MS |
In vivo Antithrombotic and Antiplatelet Activities of a Quantified Acanthopanax sessiliflorus Fruit Extract |
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| SONG Yang,YANG Chun-Juan,YU Kai,LI Fa-Mei.In vivo Antithrombotic and Antiplatelet Activities of a Quantified Acanthopanax sessiliflorus Fruit Extract[J].Chinese JOurnal of Natural Medicines,2011(2):141-145. |
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| Authors: | SONG Yang YANG Chun-Juan YU Kai LI Fa-Mei |
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| Institution: | SONG Yang,YANG Chun-Juan,YU Kai,LI Fa-Mei Department of Analytical Chemistry,Shenyang Pharmaceutical University,Shenyang 110016,China |
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| Abstract: | AIM: To investigate the in vivo antithrombotic and antiplatelet activities and triterpenoid saponin contents of ethanol extract of Acanthopanax sessiliflorus fruits (ASFEtOH). METHODS: In the present work a quantified ASFEtOH was selected to determine its in vivo antithrombotic activity in a rat arteriovenous shunt thrombosis model and its inhibitory effect on adenosine diphosphate (ADP)-induced platelet aggregation using a platelet aggregometer. Ten triterpenoid saponin contents of the extract of the fruit... |
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| Keywords: | Acanthopanax sessiliflorus fruits In vivo antithrombotic activity Antiplatelet activity Shunt thrombosis model Triterpenoid UPLC/MS/MS |
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