基于肠道菌群探讨水合橙皮内酯对AS抑郁症共病模型大鼠的调节作用

  目的  分析水合橙皮内酯(Meranzin hydrate, MH)治疗AS和抑郁共病的疗效, 探讨药物的作用机理。  方法  实验采用维持饲料饲养SD大鼠作为对照组, 高脂饲料饲养ApoE-/-大鼠12周建立AS抑郁症共病模型, 并随机分为模型组和干预组(MH3.5 mg·kg-1、MH 7 mg·kg-1和辛伐他汀7 mg·kg-1), 每组6只。  结果  MH干预能有效降低血浆和海马促炎因子TNF-α、IL-1β和IL-6水平, 调整血脂水平, 下调主动脉斑块面积, 增加海马5-羟色胺(5-Hydroxytryptamine, 5-HT)水平, 改善大鼠抑郁样行为, 调整肠道菌群结构。  结论  MH通过抗炎调脂, 调整肠道菌群结构从而发挥抗AS抑郁共病的作用。 

Meranzin Hydrate Effects Atherosclerosis and Depressive Comorbidity in ApoE-/- Rats by Antidyslipidemic,Anti-Inflammatory and Regulate Intestinal Flora

  OBJECTIVE  Focus on the efficacy of Meranzin hydrate (MH) in the treatment of comorbid AS and depression, and dicuss the mechanism of MH.  METHODS  Sprague Dawley rats with chow diets were elected as the control group, and ApoE-/- rats were fed high-fat diets for 12 weeks to establish the AS multimorbidity with depression model. ApoE-/- rats were randomly divided into the model group and intervention groups (MH 3.5 mg·kg-1, MH 7 mg·kg-1, and simvastatin 7 mg·kg-1 groups). n=6, for each group.  RESULTS  Treatment of comorbid AS and depression with MH could significantly decrease lipid levels, plaque areas, plasma level of inflammatory factors and hippocampus inflammatory cytokines, particularly tumor necrosis factor-α, interleukin(IL)-1β, and IL-6. MH could also improve hippocampal 5-hydroxytryptamine levels as well as depressive-like behavior and regulate intestinal flora.  CONCLUSION  This study demonstrates that MH reduces the inflammatory response as well as lipid levels and improves the composition of intestinal flora, which plays an important role against AS multimorbidity with depression.