miR-431-3p对胃癌细胞增殖和凋亡的影响及其靶向调控CTDP1基因表达机制

目的：探讨miR-431-3p对胃癌细胞增殖和凋亡的影响，阐明其可能的分子机制。方法：取经病理诊断确诊为胃癌的68例患者的胃癌组织及其癌旁组织。采用实时荧光定量PCR(RT-qPCR)法检测胃癌组织、癌旁组织和人正常胃黏膜上皮GES-1细胞、人胃癌细胞（MKN-28、MGC-803、 MKN-45、 SGC-7901和HGC-27）中miR-431-3p及羧基末端结构域磷酸酶1 (CTDP1)mRNA表达水平，Western blotting法检测上述细胞中CTDP1蛋白和各组SGC-7901细胞中细胞色素C(Cyt C)、B细胞淋巴瘤2 (Bcl-2)和Bcl-2相关X蛋白（Bax）蛋白表达水平。采用Pearson相关分析法分析miR-431-3p与CTDP1 mRNA表达水平的相关性，双荧光素酶报告基因实验检测miR-431-3p和CTDP1的靶向关系。将miR-431-3p mimic和CTDP1过表达慢病毒分别或同时转染至SGC-7901细胞中，将细胞分为空白组、空载过表达（mimic NC）组、miR-431-3p过表达（miR-431-3p mimic）组、空载慢病毒（Ve...

Effect of miR-431-3p on proliferation and apoptosis of gastric cancer cells and its mechanism of targeted regulation of CTDP1 gene expression

Methods The gastric cancer tissue and the adjacent tissue of 68 patients with gastric cancer confirmed by pathological diagnosis were collected.The expression levels of miR-431-3p mRNA and carboxy-terminal domain phosphatase 1 （CTDP1） mRNA in gastric cancer tissue， adjacent tissue， human normal gastric mucosal epithelial GES-1 cells and human gastric cancer cells （MKN-28， MGC-803， MKN-45， SGC-7901， HGC-27 cells ）were detected by real-time fluorescence quantitative PCR （RT-qPCR） method；the expression levels of CTDP1 protein in the above cells and the expression levels of cytochrome C（Cyt C），B cell lymphoma-2 （Bcl-2）， and Bcl-2 associated X protein （Bax） in the SGC-7901 cells in various groups were detected by Western blotting method； the correlation between miR-431-3p and CTDP1 mRNA expression level was analyzed by Pearson correlation analysis；the targeting relationship between miR-431-3p and CTDP1 was detected by dual luciferase reporter gene experiment； the miR-431-3p mimic and CTDP1 over-expression lentivirus were transfected into the SGC-7901 cells separately or at the same time.The cells were divided into blank group， vector over-expression（mimic NC） group，miR-431-3p over-expression （miR-431-3p mimic） group，vector lentivirus（vector） group， CTDP1 over-expression lentivirus （CTDP over-expression）group and miR-431-3p mimic+CTDP1 over-expression （co-transfection） group.The proliferation activities of the cells in various groups were detected by MTT assay； the clone formation numbers of the SGC-7901 cells in various groups were detected by clone formation assay；the apoptotic rates of the SGC-7901 cells in various groups were detected by flow cytometry. Results Compared with the adjacent tissue， the expression level of miR-431-3p in the gastric cancer tissue was decreased （P<0.01），and the expression level of CTDP1 mRNA was increased （P<0.01）， and there was a negative correlation between them （r=－0.316， P=0.009）. Compared with the GES-1 cells， the expression levels of miR-431-3p in the other five kinds of gastric cancer cells were decreased （P<0.01）， and the expression levels of CTDP1 mRNA and protein were increased （P<0.05）. The results of dual luciferase reporter system showed that miR-431-3p targetedly regulated the expression of CTDP1. Compared with blank group and mimic NC group， the expression levels of CTDP1 and Bcl-2 proteins， proliferation activity，and number of clone formation of the SGC-7901 cells in miR-431-3p group were decreased （P<0.05）， while the apoptotic rate， expression levels of Cyt C and Bax proteins were increased （P<0.05）. Compared with blank group and vector group， the expression levels of CTDP1 and Bcl-2 proteins， proliferation activity， and number of clone formation of the SGC-7901 cells in CTDP1 over-expression group were increased （P<0.05）， while the apoptotic rate， the expression levels of Cyt C and Bax proteins were decreased （P<0.05）. Compared with blank group and miR-431-3p group， the expression levels of CTDP1 and Bcl-2 proteins， proliferation activity，and number of clone formation of the SGC-7901 cells in co-transfection group were increased （P<0.05）， while the apoptotic rate， expression levels of Cyt C and Bax proteins were decreased （P<0.05）. Conclusion Over-expression of miR-431-3p can inhibit the proliferation and promot the apoptosis of the human gastric cancer cells， and its mechanism may be related to targeted down-regulation of the CTDP1 gene expression. Objective To discuss the effect of miR-431-3p on the proliferation and apoptosis of the gastric cancer cells，and to elucidate its possible molecular mechanism.