TB.IGRA在海分枝杆菌感染诊断和鉴别诊断中的应用

目的:探讨结核感染T细胞γ-干扰素释放实验(TB.IGRA)在海分枝杆菌感染诊断和鉴别诊断中的应用价值.方法:回顾性分析在我院门诊诊疗的疑似海分枝杆菌感染患者,排除结核病患者,根据患者海分枝杆菌qPCR是否阳性分为海分枝杆菌感染组和真菌、普通细菌感染组.应用TB.IGRA检测患者外周血中结核感染特异性T细胞,比较分析T...

Application of TB.IGRA in the diagnosis and differential diagnosis of Mycobacterium marinum infection

Objective: To investigate the application value of TB.IGRA assay in the diagnosis and differential diagnosis of Mycobacterium marinum infection. Methods: The data of suspected patients with M.marinum infection in out-patient treatment of our hospital were analyzed retrospectively. Exclusion of tuberculosis, the patients were divided into M.marinum infection group and control group (fungal and other bacterial infections) according to the qPCR result. TB.IGRA was used to detect tuberculosis infection-specific T cells of patients' peripheral blood. The sensitivity, specificity, predictive value and likelihood ratio between TB.IGRA and acid-fast staining in the diagnosis of M.marinum infection were compared and analyzed. We further discussed the sensitivity, specificity of TB.IGRA and γ- Interferon level of patients with M.marinum infection in different age groups. Results: A total of 87 patients with M.marinum infection and 25 controls were enrolled. The sensitivity and specificity of TB.IGRA assay were 73.56% and 64.0%, respectively. The positive and negative predictive value and likelihood ratio were 87.7%, 41.0%, 2.04 and 0.41, respectively. The sensitivity of young group, middle-aged group and old group were 70.0%, 73.5% and 74.4%, respectively. The specificity was highest in the middle-aged group (88.9%) and lowest in the elderly group (46.2%).  Conclusion: TB.IGRA has important application value in the diagnosis and differential diagnosis of M.marinum infection.