1株珊瑚共附生真菌Talaromyces verruculosus GXIMD 02504的次级代谢产物及抑菌活性研究

摘要：目的 开展1株北部湾来源珊瑚共附生真菌Talaromyces verruculosus GXIMD 02504的次级代谢产物及其抗菌活性研 究。方法 采用多种现代色谱分离技术对菌株发酵产物进行分离纯化，利用核磁共振技术和文献数据对比解析化合物的化学 结构，并采用滤纸片琼脂扩散法和改良肉汤稀释法测定其抑菌活性。结果 从其液体发酵产物中共分离鉴定了9个化合物，分 别为chrodrimanins A-B, E-F, H(1~5)、芝麻素(6)、樱黄素(7)、5,7,4-三羟基异黄酮(8)、N-acetyl-tryptamine(9)。化合物8显示出对 表皮葡萄球菌(Staphylococcus epidermidis)的弱抑制活性，最小抑菌浓度(minimal inhibitory concentration, MIC)为78 μg/mL。结 论 从菌株T. verruculosus GXIMD 02504中分离得到以chrodrimanin类混源萜为主的化合物9个，其中化合物6~8为首次从该属中 分离得到，且化合物8具有一定的抗表皮葡萄球菌活性。本研究丰富了该菌次级代谢产物的结构多样性。

Antibacterial secondary metabolites from a coral-derived fungus Talaromyces verruculosus GXIMD 02504

Abstract Objective To investigate the secondary metabolites from the Beibu Gulf coral-derived fungus Talaromyces verruculosus GXIMD 02504 and their antibacterial activity. Methods The fermentation products of the strain were isolated and purified by various modern chromatographic separation techniques. Structures of the obtained compounds were identified by nuclear magnetic resonance data analysis as well as comparison of literature data. Their antibacterial activity was further evaluated by methods of the Kirby-Bauer test and the modified broth dilution. Results Nine compounds (1~9) were obtained from the liquid fermentation products, which were determined as chrodrimanins A~B, E~F, H (1~5), sesamin (6), prunetin (7), 5,7, 4-trihydroxyisoflavone (8), and N-acetyl-tryptamine (9), respectively. Compound 8 showed weak antibacterial activity against Staphylococcus epidermidis, with minimal inhibitory concentration (MIC) of 78 μg/mL. Conclusion Nine compounds exemplified by chrodrimanin-typemeroterpenoids were isolated from Talaromyces verruculosus GXIMD 02504, and meanwhile compounds 6~8 were obtained from the Talaromyces genus for the first time. In addition, compound 8 was found with antibacterial activity against S. epidermidis. This study would enrich the structural diversity of the strain.