虫草素抑制食管癌细胞Eca-109增殖、迁移和侵袭的作用及机制研究

目的:探讨虫草素对食管癌细胞Eca-109的作用及其潜在的机制。方法:不同浓度（35、70、140、280 μg/mL）虫草素分别处理Eca-109细胞24 h、48 h、72 h和96 h，CCK-8检测细胞活力，筛选虫草素最佳作用浓度（70 μg/mL）和时间（24 h）。实验分为4组:对照组、虫草素组（70 μg/mL虫草素）、SB203580组（10 μg/mL SB203580）和虫草素+SB203580组（70 μg/mL虫草素+10 μg/mL SB203580）。培养24 h后，CCK-8检测细胞增殖能力；流式细胞术检测细胞周期和凋亡；Transwell小室检测细胞迁移和侵袭能力；qRT-PCR和Western blot分别检测细胞周期相关、凋亡相关和p38 MAPK信号通路相关基因和蛋白的表达。结果:与对照组相比，虫草素组、SB203580组和虫草素+SB203580组细胞增殖、迁移和侵袭能力显著降低（P＜0.05），细胞发生G2期阻滞，凋亡率显著升高（P＜0.05），cyclinB1、CDK4、p-p38、ERK1和ERK2的表达下调（P＜0.05），CKI和Caspase-3的表达上调（P＜0.05）。结论:虫草素可抑制食管癌细胞Eca-109增殖、迁移和侵袭，促进细胞凋亡，其作用机制与p38 MAPK信号通路有关。

Effect and mechanism of cordycepin on inhibition of proliferation,migration and invasion of esophageal carcinoma cells Eca-109

Objective:To investigate the effect of cordycepin on esophageal carcinoma cells Eca-109 and its potential mechanism.Methods:Eca-109 cells were treated with different concentrations of cordycepin (35,70,140 and 280 μg/mL) for 24 h,48 h,72 h and 96 h,respectively.The cell viability was detected by CCK-8 kit to screen the optimal concentration (70 μg/mL) and time (24 h) for the cordycepin.The experiment was divided into 4 groups:Control group,cordycepin group (70 μg/mL cordycepin),SB203580 group (10 μg/mL SB203580) and cordycepin+SB203580 group (70 μg/mL cordycepin+10 μg/mL SB203580).After 24 h of culture,CCK-8 kit assayed cell proliferation ability.Flow cytometry assayed cell cycle and apoptosis.Transwell assayed cell migration and invasion ability.qRT-PCR and Western blot assayed cell cycle-related,apoptosis-related and p38 MAPK signaling pathway-related gene and protein expression,respectively.Results:Compared with the control group,cell proliferation,migration and invasion ability were significantly reduced (P＜0.05),and cells underwent G2 phase block,and apoptosis rate was significantly increased (P＜0.05),expression of cyclinB1,CDK4,p-p38,ERK1 and ERK2 was downregulated (P＜0.05),and the expression of CKI and Caspase-3 was upregulated (P＜0.05) in the cordycepin group,SB203580 group and cordycepin+SB203580 group.Conclusion:Cordycepin inhibits the proliferation,migration and invasion and promotes apoptosis in esophageal carcinoma cells Eca-109,and its mechanism was related to the p38 MAPK signaling pathway.