电针干预对透镜诱导型近视豚鼠视网膜和视皮层中BDNF、NGF及AchE表达的影响

目的 探讨电针干预对透镜诱导型近视(LIM)豚鼠视网膜和视皮层中脑源性神经营养因子(BDNF)、神经生长因子(NGF)及乙酰胆碱酯酶(AchE)表达变化的影响.方法 将90只2周龄雄性豚鼠随机分为正常对照(NC)组、LIM组和LIM+电针治疗(LIM+EA)组.NC组豚鼠正常饲养,不进行干预;LIM组和LIM+EA组豚...

Effect of electroacupuncture on the expression of brain-derived neurotrophic factor,nerve growth factor and acetylcholine esterase in retina and visual cortex of guinea pigs with lens-induced myopia

Objective　To investigate the effect of electroacupuncture (EA) on the expression of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), and acetylcholine esterase (AchE) in the retina and visual cortex of guinea pigs with lens-induced myopia (LIM).Methods　Ninety two-week-old male guinea pigs were randomly divided into the normal control (NC) group, LIM group, and LIM+EA group. Guinea pigs in the NC group were bred normally without intervention, while guinea pigs in the LIM and LIM+EA groups were fitted with -6.0 D lenses on the right eye, and the left eye was used as self-control to establish a myopia model. Besides, guinea pigs in the LIM+EA group were given EA at the Temple and Hegu acupoints while wearing the lens. After 2 and 4 weeks of modeling, the diopter and axial length of guinea pigs in each group were measured, the mRNA and protein expression levels of BDNF, NGF, and AchE were determined by the quantitative polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assay (ELISA), and the AchE vitality was detected by AchE assay kits.Results　After 2 and 4 weeks of modeling, in terms of the difference between the model eye (right eye) and the self-control eye (left eye), the diopter and axial length in the LIM group were significantly increased compared with the NC group (both P<0.01), while the diopter and axial length in the LIM+EA group were significantly decreased compared with the LIM group (both P<0.05). qPCR and ELISA results showed that after 2 and 4 weeks of modeling, the mRNA and protein levels of BDNF and NGF in the retina and visual cortex of the right eye in the LIM group were significantly reduced compared with the NC group, while the mRNA and protein levels of AchE were significantly increased (all P<0.05). Compared with the LIM group, the mRNA and protein levels of BDNF and NGF in the retina and visual cortex of the right eye in the LIM+EA group were significantly increased, while the mRNA and protein levels of AchE were significantly decreased (all P<0.05). AchE assay results showed that after 2 and 4 weeks of modeling, the AchE vitality in the retina and visual cortex of the right eye in the LIM group was significantly higher than that in the NC group (all P<0.01). The AchE vitality in the retina and visual cortex of the right eye in the LIM+EA group was significantly lower than that in the LIM group (all P<0.01).Conclusion　The BDNF and NGF levels in the retina and visual cortex of guinea pigs with myopia are decreased, while the AchE level is increased. EA can significantly elevate the expression levels of BDNF and NGF and decrease the expression level of AchE in myopic guinea pigs, thereby delaying the development of myopia.