基于液质联用技术的南北产地桔梗药材皂苷谱差异研究

  目的  通过鉴定9个省(市)的57批桔梗药材中皂苷类成分, 绘制并比较南北产地桔梗药材皂苷谱。  方法  以UPLC-LTQ-Orbitrap-MS和LC-MS/MS法测定桔梗药材中皂苷类成分, 采用主成分分析(PCA)及正交偏最小二乘法判别分析(OPLS-DA)处理样品数据。  结果  桔梗药材中共鉴定出48种皂苷, PCA及OPLS-DA均显示南北桔梗药材中皂苷有较好区分, 共发现23种差异皂苷。靶标定量结果显示南桔梗中桔梗皂苷D、去芹糖桔梗皂苷D、远志皂苷D及去芹糖桔梗皂苷D₂含量显著高于北桔梗。  结论  南北桔梗药材在皂苷成分上有着明显差异, 同时建立了快速、稳定的靶标定量方法, 可为桔梗药材质量控制提供参考。 

Study on the Difference of Saponins Profile of Platycodonis Radix from the Northern and Southern Regions Based on Liquid Chromatography-Mass Spectrometry

  OBJECTIVE  To identify the spanions of 57 batches of Platycodonis Radix from 9 provinces and cities, draw and compare the saponins profile between southern and northern platycodons.  METHODS  UPLC-LTQ-Orbitrap-MS and LC-MS/MS were used to determine the saponins in Platycodonis Radix. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to analyze data.  RESULTS  A total of 48 saponins were identified in Platycodonis Radix. Both PCA and OPLS-DA showed that the northern and southern samples were well distinguished, 23 different saponins were found. Target quantitative results showed that the contents of platycodin D, deapio-platycodin D, polygalacin D and deapio-platycodin D₂ of southern samples were significantly higher than those of northern samples.  CONCLUSION  There are significant differences in saponins between the northern and southern Platycodonis Radix, and the target quantification method is fast and stable, which can provide references for the quality control of Platycodonis Radix.