嗜酒前后胃乙醇脱氢酶活性变化机制研究

目的探讨嗜酒前后胃乙醇脱氢酶(ADH)活性的变化机制。方法Wistar大鼠39只随机分成两组:模型组24只,对照组15只,模型组给予乙醇灌胃,对照组用蒸馏水灌胃;用HE病理染色技术和酶组化电镜染色技术,观察给酒前后胃黏膜的细胞及亚细胞结构的变化。结果模型组给酒后,HE病理显示胃粘膜的主细胞、壁细胞等明显肿胀变性,酶组化电镜显示主细胞和壁细胞的内质网和线粒体发生溶解;对照组实验前后未发生上述改变。结论给酒前后胃乙醇脱氢酶活性变化的机制可能是由于胃黏膜的主细胞、壁细胞水肿变性,从而导致其线粒体和内质网变性、溶解有关。

Study on Mechanism of Activities of Gastric Alcohol Dehydrogenases before and after Abetiuence of Alcohol

Objective To study the mechanism of activity change of gastric alcohol dehydrogenases(ADH).We investigated ADH before and after ingestion of alcohol on rats.Methods Thirty-nine rats were divided into two groups at random:model ones(24 cases) and control ones(15 cases).Model group of rats were infused into stomaches by alcohol,and control ones by distill water.Structures of cell and sub-cell in gastric mucous membrane were observed by enzyme electron microscope and hematoxylin eosin(HE) stain.Results It was shown that there were dropsical degeneration of chief cells and parietal cells of stomaches by HE stain,and the mitochondria of parietal cells and the rough-surfaced endoplasmic reticulum(rER) of chief cells were destroyed by enzyme electron microscope in model groups after two months of ethanol perfusion.There were not change in control group.Conclusions The mechanism of activity alterations of gastric ADH may result from dropsical degeneration of chief cells and parietal cells of stomach,which result in degeneration,solution and breakage of construction of rER in the chief cells and mitochondria in parietal cells.