| MiRP1对异源转染的CHO细胞上HCN1和HCN2通道电生理特性的调节 |
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| 引用本文： | 佘强,邓松柏,Uta C.Hoppe.MiRP1对异源转染的CHO细胞上HCN1和HCN2通道电生理特性的调节[J].第三军医大学学报,2008,30(12):1129-1131. |
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| 作者姓名： | 佘强 邓松柏 Uta C.Hoppe |
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| 作者单位： | 1. 重庆医科大学附属第二医院心血管内科,重庆,400010
2. 德国科隆大学医院心血管内科,德国科隆,50924 |
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| 摘 要： | 目的 评价Mink相关肽1(MiRP1)对异源转染的CHO细胞上的HCN1、HCN2通道电生理特性的影响.方法 将MiRP1质粒DNA分别和HCN1、HCN2质粒DNA共转染CHO-K1细胞,用全细胞膜片钳记录细胞膜上的HCN通道电流.结果 MiRP1显著增加HCN2 (37.8±4.8) pA/pF(n=11) vs (25.9±1.7) pA/pF(n=10); -140 mV, P<0 05]的电流密度,但是对HCN1的电流大小没有影响(41.3±10.9) pA/pF(n=13) vs (34.9±7.8) pA/pF(n=11); P>0 05];MiRP1可加快HCN1时间常数:(180.9±8.6) ms vs (306.1±45.6) ms; -80 mV, P<0 05]和HCN2 时间常数:(391.8±33.6) ms vs (763.5±106.1) ms; -80 mV, P<0 05]激活动力学;MiRP1对HCN1及HCN2通道激活的电压依赖性没有影响.结论 MiRP1可加快HCN1和HCN2通道激活,且增加HCN2的电流表达.
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| 关 键 词： | HCN1通道 HCN2通道 膜片钳 Mink相关肽1 |
| 文章编号： | 1000-5404(2008)12-1129-03 |
| 修稿时间： | 2007年11月30 |
MiRP1 regulates electrophysiological properties of HCN1 and HCN2 in CHO cells in vitro |
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| SHE Qiang,DENG Song-bai,Uta C.Hoppe.MiRP1 regulates electrophysiological properties of HCN1 and HCN2 in CHO cells in vitro[J].Acta Academiae Medicinae Militaris Tertiae,2008,30(12):1129-1131. |
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| Authors: | SHE Qiang DENG Song-bai Uta CHoppe |
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| Institution: | SHE Qiang1,DENG Song-bai1,Uta C.Hoppe2 (1 Department of Cardiology,Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China,2Department of Cardiology,Cologne University,Cologne 50924,Germany) |
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| Abstract: | Objective To investigate the modulation of electrophysiological properties of hyperpolarization-activated cyclic nucleotide-gated channels(HCN) HCN1 and HCN2 by transfecting MinK-related peptide 1(MiRP1) in Chinese hamster ovarian(CHO) cells.Methods CHO cells were co-transfected with plasmid DNA encoding either of the cardiac HCN isoforms(HCN1,HCN2) with MiRP1 to observe the effect of MiRP1 on HCN whole-cell currents.Whole-cell patch clamp technique was used to record the 2 channel currents of the transfect... |
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| Keywords: | hyperpolarization-activated cyclic nucleotide-gated channels 1 hyperpolarization-activated cyclic nucleotide-gated channels 2 patch clamp MinK-related peptide 1 |
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