In vitro stimulation with mitogen and antigen in patients with monoclonal gammopathy |
| |
| Authors: | O v Hutten Priv Doz Dr A Fateh-Moghadam H Grosse-Wilde W Mempel B Netzel W Ruppelt |
| |
| Institution: | (1) Abteilung Immunologie der GSF München, Institut für Hämatologie, Germany;(2) Bereich Protein- und Immunchemie, Institut für Klinische Chemie am Klinikum Großhadern der Universität München, Germany;(3) Klinikum Mannheim der Universität Heidelberg I. Medizin, 6800 Mannheim 1, Germany |
| |
| Abstract: | Summary Peripheral blood lymphocytes from 32 patients with defined paraproteinaemia (16 IgG, 9 IgA and 7 IgM) and from 15 healthy donors were studied for their in vitro response to various stimuli, including unspecific mitogens such asPhytohaemagglutinin (PHA),Pokeweedmitogen (PWM) and Concanavalin A (ConA) as well as specific antigens such as purified Tuberculin, Candida, Varidase, Tetanus Toxoid, Vaccinia antigen and Vaccinia-control antigen.Mitogens and antigens were lyophilized in Microtiter plates. The lymphocytes of all tested patient-groups responded (measured by H3-Thymidin-up-take) significantly lower towards the unspecific mitogens than those of the control group. If the patients' lymphocytes were stimulated by the specific antigens, their in vitro response was significantly diminished to candida and vaccinia. Macroglobulinaemia showed significantly lower response to ConA if compared to myelomas of IgG- and IgA-type. No correlation was found between mitogen and antigen response and the serum concentration of the paraproteins or immunoglobulins. The results show that monoclonal gammopathy and especially macroglobulinaemia are associated with abnormalities of the cellular immunity which correlates with the clinical observation of increased fungal and viral infections.Supported in part by SFB 37 München and Euratom/GSF BIAD I 031-64 |
| |
| Keywords: | Lymphocytes stimulation Monoclonal gammopathy Cellular immunity |
| 本文献已被 SpringerLink 等数据库收录！ |
|
