整合素αvβ3在肝星状细胞和肝纤维化组织中的表达

目的 研究整合素avβ3在肝星状细胞和纤维化肝组织中的表达,探讨能反映肝星状细胞活化状态的表型受体.方法 分离大鼠肝星状细胞,体外培养激活.硫代乙酰胺175 mg/kg每周2次,连续12周腹腔注射诱导大鼠肝纤维化.免疫染色观察细胞和组织中整合avβ3和a-平滑肌肌动蛋白(a-SMA)的表达.实时PCR和Western印迹法分析avβ3在星状细胞中、正常和肝纤维化组织中的表达.结果 活化星状细胞中avβ3表达明显上调,与第1天相比,第14天的mRNA和蛋白质水平分别增加18倍和5.2倍.免疫染色整合素avβ3成簇极性表达于活化星状细胞的胞膜.肝纤维化模型中avβ3在mRNA和蛋白水平明显高于正常对照(t=2.39,P＜0.05;t=2.74,P＜0.05),正常肝组织中a-SMA和avβ3局限表达于门静脉周围,肝纤维化组织中a-SMA和avβ3表达于扩大的门管区和纤维间隔.结论 体内外活化星状细胞中整合素avβ3表达水平上调,它是星状细胞活化的表型受体,参与肝纤维化的发生.

Expression of integrin avβ3 in hepatic stellate cells and fibrotic liver tissue

Objective To investigate the expression of integrin avβ3 in both of hepatic stellate cell (HSC)and fibrotic liver tissue,and to demonstrate whether integrin avβ3 is a phenotypical receptor of activated HSC.Methods HSC were isolated from Sprague-Dawley rats and activated by prolonged cultural.The rats were injected with 175 mg of thioacetamide twice a week for 12 weeks to induce liver fibosis.The expressions of avβ3 and a-SMA were identified by immunocytochemical staining.The expression of avβ3 in HSC,normal and fibrotic tissues were examined by real-time PCR and Western blot.Results The expression of avβ3 in activated HSC were up regulated and levels of mRNA and protein were increased to 18 and 5.2 folds on day 14 compared with day 1 and were also higher than control(t=2.39,P＜0.05;t=2.74,P＜0.05).The immunochemistry staining showed that integrin avβ3 was expressed on membrane of activated HSCs.The avβ3 and a-SMA were expressed in portal vein in normal liver,but were also expressed in portal and fibrotic.Conclusions The integrin avβ3 is up-regulated in activated HSC both in vitro and in vivo.It is a phenotypical receptor of activated HSC which involved in liver fibrosis.