复合氨基酸注射液(17AA)中N—乙酰—L—酪氨本通讯N—乙酰—L—半胱氨酸的HPLC测定 |
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引用本文: | 高春,纪宏,高恒莹,姚瑛.复合氨基酸注射液(17AA)中N—乙酰—L—酪氨本通讯N—乙酰—L—半胱氨酸的HPLC测定[J].中国医药工业杂志,2001,32(2):65-66. |
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作者姓名: | 高春 纪宏 高恒莹 姚瑛 |
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作者单位: | 北京市药品检验所, |
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摘 要: | 对复合氨基酸注射液(17AA)中含量较低的N-乙酰-L-酪氨酸及N-乙酰-L-半胱氨酸用HPLC法进行测定,采用阳离子交换色谱柱,以水(取0.05mol/L硫酸1ml置1000ml量瓶中以水定容,调节pH至3.0)为流动相,检测波长为210nm。该方法在10-100ug/ml范围呈良好的线性,相关系数分别为0.9996和0.9999,平均回收率分别为99.94%和99.72%,RSD分别为0.42%及1.19%。
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关 键 词: | N-乙酰-L-酪氨酸 N-乙酰-L-半胱氨酸 HPLC 测定 复方氨基酸注射液 |
文章编号: | 1001-8255(2001)02-0065-02 |
修稿时间: | 1999年9月14日 |
Determination of
N-Acetyl-L-tyrosine and N-Acetyl-L-cysteine in Compound Amino Acids Injection (17AA) by
HPLC |
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Authors: | Gao Chun JI Hong GAO Heng-Ying Yao Ying |
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Abstract: | A HPLC method for determination of N-acetyl-L-tyrosine and N-acetyl-L-cysteine was established using Supelcosil LC-SCX column with the mobile phase of water (pH 3.0), at the detection wavelength of 210 nm. They had good linearites in the range of 10~100 μg/ml, with r of 0.9996 and 0.9999, respectively. The average recoveries of N-acetyl-L-tyrosine and N-acety-L-cysteine were 99.94% and 99.72% with RSD of 0.42% and 1.19%, respectively. |
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Keywords: | N acetyl L tyrosine N acetyl L cysteine HPLC determination |
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