| 重组人p53腺病毒药物对人喉癌细胞的抑制实验 |
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| 引用本文： | 敖敏,何刚,梁传余. 重组人p53腺病毒药物对人喉癌细胞的抑制实验[J]. 现代预防医学, 2007, 34(6): 1034-1035 |
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| 作者姓名： | 敖敏 何刚 梁传余 |
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| 作者单位： | 1. 四川省人民医院耳鼻喉科,成都,610000
2. 四川大学华西医院耳鼻喉科 |
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| 摘 要： | [目的]探索p53基因在喉癌基因治疗方面的可行性。[方法]以人喉癌细胞系Hep-2为实验对象,将重组人p53腺病毒药物(rAd/p53)转染Hep-2细胞,体外实验观察重组人p53腺病毒药物(rAd/p53)对Hep-2细胞生长的影响。[结果]各浓度重组人p53腺病毒药物(rAd/p53)(1010、109、108、107)对Hep-2生长均有抑制。尤以1010明显。转染3d后,重组人p53腺病毒药物(rAd/p53)诱导Hep-2细胞明显凋亡。[结论]重组人p53腺病毒药物(rAd/p53)对Hep-2细胞生长能有效抑制,能明显诱导其凋亡,为喉癌的治疗提供了临床前依据。
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| 关 键 词： | 喉癌细胞 重组人p53腺病毒 基因治疗 凋亡 |
| 文章编号： | 1003-8507（2007）06-1034-02 |
| 收稿时间： | 2006-03-10 |
| 修稿时间： | 2006-03-10 |
ADENOVIRUS-MEDIATED P53 GENE THERAPY OF HUMAN LARYNGEAL CANCER |
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| AO Min,HE Gang,LIANG Chuan-yu. ADENOVIRUS-MEDIATED P53 GENE THERAPY OF HUMAN LARYNGEAL CANCER[J]. Modern Preventive Medicine, 2007, 34(6): 1034-1035 |
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| Authors: | AO Min HE Gang LIANG Chuan-yu |
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| Abstract: | [Objective]To explore the feasibility of p53 in gene therapy for laryngeal cancer.[Methods]A human laryngeal cancer cell line Hep-2 was used.Adenoviral-mediated transfer of p53 gene(rAd/p53)was transiently introduced into Hep-2 cells in vitro.The growth of Hep-2 cells in vitro was examined.[Results]Cell growth was greatly suppressed in vitro studies.Apoptosis began obviously on the 4th day after rAd/p53 infection.[Conclusions]rAd/p53 is a potential approach to the therapy of laryngeal cancer. |
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| Keywords: | Laryngeal neoplasms Adenovirus-mediated P53 Gene therapy Apoptosis |
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