淫羊藿苷对大鼠颈动脉损伤后新生内膜增生和平滑肌细胞凋亡的影响

目的:研究淫羊藿苷对大鼠颈动脉损伤后新生内膜增生和平滑肌细胞凋亡的影响。方法:大鼠44只随机分为正常对照组8只,淫羊藿苷60 mg.kg-1组、淫羊藿苷120 mg.kg-1组和模型组各12只。后3组建立鼠颈动脉球囊损伤术后狭窄模型,淫羊藿苷组术前6 d开始ig至实验结束;模型组以等体积生理盐水ig。术后14 d和28 d分批处死动物。取各组动物颈动脉损伤段,常规制片,HE染色,采用计算机图像分析系统测量内膜面积和中膜面积,并计算新生内膜/中膜面积比(I/M);采用末端脱氧核苷酸转移酶介导的三磷酸脱氧尿嘧啶缺口末端标记法(TUNEL)检测平滑肌细胞凋亡。结果:给药两组术后14,28 d,损伤部位血管内膜、中膜面积明显小于模型组,差异非常显著(P0.01);球囊损伤后14,28 d,各手术组新生内膜区均出现明显的凋亡细胞,其中淫羊藿苷组较模型组细胞凋亡率显著增高(P0.01),结论:淫羊藿苷可能通过促进平滑肌细胞的凋亡而减轻球囊损伤后新生内膜的增生。

Effect of Icariin on Intimal Proliferation and Apoptosis of Vascular Smooth Muscle Cell after Vascular Injury in Rats

Objective: To investigate the effect of Icariin (ICA) on intimal proliferation and apoptosis of vascular smooth muscle cell after vascular injury in rat. Method : Fourty-four rats were randomly divided into 4 groups: natural group,model group and ICA 60,120 mg ·kg^-1 group.Rat model of carotid restenosis induced by balloon injury was established. Rats of ICA group were treated with drugs for 2 or 4 weeks. Then the neointimal area(IA),medial area (MA), and neointimal area(IA)/medial area(MA) were analyzed by computerized digitizer system. TUNEL method was used to detected the apoptosis in the artery. Result :At each time point, the lumem area was larger and the neointima area was smaller in all the treated groups compared with that in model group (P<0.01). The expression of vascular smooth muscle cell apoptosis in the vascular in all the treated groups was increased, that of the model group (2 w, 4 w) being the lowest (P <0.01). Conclusion : ICA can effectively repress neointimal proliferation after vascular injury, The effect may be associated with its action of promoting apoptosis of vascular smooth muscle cells.