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| 凋亡调节蛋白的表达与K562／VCR多药耐药性的关系 | |
| 引用本文： | 朱玉胜,吕元,林果为,张荣兴,杨正洪,吕沅冈.凋亡调节蛋白的表达与K562／VCR多药耐药性的关系[J].复旦学报(医学版),2001,28(1):32-34. |
| 作者姓名： | 朱玉胜 吕元 林果为 张荣兴 杨正洪 吕沅冈 |
| 作者单位： | 1. 复旦大学附属华山医院检验科 2. 复旦大学医学院附属华山医院血液科 3. 中国科学院细胞库 |
| 基金项目： | 上海市卫生局“百人计划”资助课题 |
| 摘 要： | 目的探讨K562/VCR细胞中凋亡调节蛋白表达的变化与其多药耐药性(MDR)的关系。方法通过免疫细胞化学法和免疫印迹法(westernblot)对凋亡调节蛋白Bcl-2、Bcl-X |
| 关 键 词： | 多药耐药性 白血病 凋亡调节蛋白 K562／VCR |
| 修稿时间： | 2000年3月29日 |
Relationship Between Expression of Apoptosis-modulating Proteins and Multidrug Resistance in K562/VCR Cells |
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| ZHU Yu-sheng,LU Yuan,LIN Guo-wei,ZHANG Rong-xing,YANG Zheng-hong,Lu Yuan-gang.Relationship Between Expression of Apoptosis-modulating Proteins and Multidrug Resistance in K562/VCR Cells[J].Fudan University Journal of Medical Sciences,2001,28(1):32-34. | |
| Authors: | ZHU Yu-sheng LU Yuan LIN Guo-wei ZHANG Rong-xing YANG Zheng-hong Lu Yuan-gang |
| Abstract: | Purpose To explore the Relationship between expression of apoptosis-modulating proteins amdmultidrug resistance in K562/VCR cells. Methods Irnmunocytochemical methol and western blot wereused to analyze the expression of apoptosis-modulating proteins (Bcl - 2, Bcl-XL, Bax, Bak ) in multidrugresistant cell line K562/VCR and drugsensitive cell line K562. Results The positive cell rates ofapoptosis-suppressing protein Bcl-2 and Bcl-XL in K562/VCR were (40.0 ± 8.0) % and (60.0 ± 10.0) % .While the rates in K562 were (1.0 ± 0.3) % and (20.0 ± 4.0) %. There was significant difference in thepositive cell rates of Bcl - 2 and Bcl - XL between K562/VCR and K562 ( n = 3, P ＜ 0.05 ). It was alsofound there was no significant difference in expression of Bax between K562/VCR and K562. Furthemore,Bak was not expressed in both K562/VCR and K562 or the expression was very low. Conclusions Wesuggest that Bcl-2 and Bcl-XL play important roles in multidrug resistance in K562/VCR, while Bax and Bakmight not be important. |
| Keywords: | multidrug resistance leukemia apoptosis modulating proteins |
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