毛连菜总酚含量测定和纯化工艺研究

目的 建立毛连菜总酚的含量测定方法，并优选其大孔吸附树脂纯化工艺。方法 采用紫外-可见分光光度法测定毛连菜总酚的含量，以吸附量、解吸率、解吸量等指标优选毛连菜总酚的大孔吸附树脂富集纯化工艺。结果 所建立的质量控制方法的精密度、重复性和稳定性良好，其RSD均<3.0%。优选出了HP-20树脂对毛连菜总酚有较好的吸附、解吸性能，最佳工艺：上样的毛连菜水溶液总酚浓度为8.54 mg·mL^-1，上样体积为1 BV，控制流速为2 BV·h^-1。先用水和10%乙醇洗去杂质，用量各4 BV，收集4 BV的30%乙醇和3 BV的50%乙醇洗脱液，浓缩，即得。结论 本实验建立的质量控制方法简单、准确，经HP-20树脂分离纯化，毛连菜总酚的纯度可达87%以上，且工艺稳定。

Determination and Purification Technology of Total Phenols from Picris Japonica Thunb.

OBJECTIVE To establish a method for the content determination and purification technology of total phenols from Picris japonica Thunb. METHODS The content of total phenols was determined by UV-visible spectrophotometry and the purification technology of total phenols by macroporous resin was optimized with extent of adsorption, adsorption rate, desorption amount and desorption rate as index. RESULTS The RSD of precision, reproducibility and stability of the quality control method were <3.0%. HPD-20 macroporous resin showed good purified capability to total phenols from Picris japonica Thunb.. Optimum technology conditions were determined as follow: 1 BV of sample solution (the content of total phenols was 8.54 mg·mL^-1) was passed through microporous resin with elution rate of 2 BV·h^-1, after eluted with 4 BV of water and 4 BV of 10% ethanol, then collected 4 BV of 30% ethanol eluent and 3 BV of 50% ethanol eluent, and concentrated the combined eluent under vacuum to yield the purified total phenols. CONCLUSION The method established for determined the content of total phenols from Picris japonica Thunb. is convenient and accurate. After used HP-20 resin and the optimized technology, the purity of total phenols from Picris japonica Thunb. is >87%, and the process is stable.