Repair of sheep metatarsus defects by using tissue-engineering technique |
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Authors: | Li Zhanghua Yang Yi Wang Changyong Xia Renyun Zhang Yufu Zhao Qiang Liao Wen Wang Yonghong Lu Jianxi |
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Institution: | 1. Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030 , China;Tissue Engineering Research Center of Academy of Military Medical Sciences, Beijing 100085, China 2. Wuhan Institute of Physical Education, Wuhan 430079, China 3. Tissue Engineering Research Center of Academy of Military Medical Sciences, Beijing 100085, China 4. Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030 , China |
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Abstract: | Summary Tissue-engineering bone with porous β-tricalcium phosphate (β-TCP) ceramic and autologous bone marrow mesenchymal stem cells
(MSC) was constructed and the effect of this composite on healing of segmental bone defects was investigated. 10–15 ml bone
marrow aspirates were harvested from the iliac crest of sheep, and enriched for MSC by density gradient centrifugation over
a Percoll cushion (1.073 g/ml). After cultured and proliferated, tissue-engineering bones were constructed with these cell
seeded onto porous β-TCP, and then the constructs were implanted in 8 sheep left metatarsus defect (25 mm in length) as experimental
group. Porous β-TCP only were implanted to bridge same size and position defects in 8 sheep as control group, and 25 mm segmental
bone defects of left metatarsus were left empty in 4 sheep as blank group. Sheep were sacrificed on the 6th, 12th, and 24th
week postoperatively and the implants samples were examined by radiograph, histology, and biomechanical test. The 4 sheep
in blank group were sacrificed on the 24th or histologically at the defects of experimental group as early as 6th week postoperatively,
but not in control group, and osteoid tissue, woven bone and lamellar bone occurred earlier than in control group in which
the bone defects were repaired in “creep substitution” way, because of the new bone formed in direct manner without progression
through a cartilaginous intermediate. At the 24th week, radiographs and biomechanical test revealed an almost complete repair
of the defect, of experimental group, only partly in control group. The bone defects in blank group, were non-healing at the
24th week. It was concluded that engineering bones constructed with porous β-TCP and autologous MSC were capable of repairing
segmental bone defects in sheep metatarsus beyond “creep substitution” way and making it, healed earlier. Porous β-TCP being
constituted with autologous MSC may be a good option in healing critical segmental bone defects in clinical practice and provide
insight for future clinical repair of segmental defect.
LI Zhanghua, male, born in 1973, M.D., Ph.D.
This project was supported by national high technology research and development program of China (863 Program, 2001AA216031),
key technologies research and development program of Beijing (H020920050031). |
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Keywords: | tissue-engineering bone bone defect regenerated new bone |
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