Association of MDR1 gene polymorphisms
with the risk of hepatocellular carcinoma in the Chinese Han
population |
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Authors: | Jian Gao |
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Institution: | Tianjin Medical University, Tianjin, People''s Republic of China |
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Abstract: | The multidrug resistance 1 gene (MDR1) is an important candidate
gene for influencing susceptibility to hepatocellular carcinoma (HCC). The
objective of the present study was to evaluate the association of
MDR1 polymorphisms with the risk of HCC in the Chinese Han
population. A total of 353 HCC patients and 335 healthy subjects were enrolled
in the study. Polymerase chain reaction-restriction fragment length polymorphism
(PCR-RFLP), created restriction site-PCR (CRS-PCR) and DNA sequencing methods
were used to identify MDR1 gene polymorphisms. Two allelic
variants (c.335T>C and c.3073A>C) were detected. The CC genotype of the
c.335T>C polymorphism was associated with an increased risk of developing HCC
compared to the TT genotype (OR = 2.161, 95%CI = 1.350-3.459, χ2 =
10.55, P = 0.0011). The risk of HCC was significantly higher for the CC genotype
in the c.3073A>C polymorphism compared to the AA genotype in the studied
populations (CC vs AA: OR = 2.575, 95%CI = 1.646-4.028,
χ2 = 17.64, P < 0.0001). The C allele of the c.335T>C and
c.3073A>C variants may contribute to the risk of HCC (C vs T
of c.335T>C: OR = 1.512, 95%CI = 1.208-1.893, χ2 = 13.07, P =
0.0003, and C vs A of c.3073A>C: OR = 1.646, 95%CI =
1.322-2.049, χ2 = 20.03, P < 0.0001). The c.335T>C and
c.3073A>C polymorphisms of the MDR1 gene were associated
with the risk of occurrence of HCC in the Chinese Han population. Further
investigations are needed to confirm these results in larger different
populations. |
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Keywords: | Hepatocellular carcinoma Multidrug resistance 1 gene Single nucleotide polymorphisms Susceptibility Risk factors |
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