Evaluation of GeneXpert and liquid culture for detection of Mycobacterium tuberculosis in pediatric patients |
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| Affiliation: | 1. Department of Microbiology and Immunology, SMS Medical College, Jaipur, Rajasthan, India;2. Department of Zoology, University of Rajasthan, Jaipur, Rajasthan, India;3. International Centre for Excellence in Laboratory Training (ICELT), National Tuberculosis Institute, Bengaluru, India;1. University of Health Sciences, Haydarpasa Numune Training and Research Hospital, Laboratory of Medical Microbiology, Istanbul, Turkey;2. University of Health Sciences, Hamidiye Medical Faculty, Department of Medical Microbiology, Istanbul, Turkey;3. Istanbul University-Cerrahpasa, Cerrahpasa School of Medicine, Department of Medical Microbiology, Istanbul, Turkey;1. Department of Laboratory Medicine, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, 210008, China;2. Department of Rheumatology and Immunology, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, 210008, China;3. Department of Infectious Diseases, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing, Jiangsu Province, 210008, China;1. Department of Experimental Animal Facility, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Agra, Uttar Pradesh, 282004, India;2. Clinical Division, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Agra, Uttar Pradesh, 282004, India;3. Department of Biochemistry, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Agra, Uttar Pradesh, 282004, India;4. National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Agra, Uttar Pradesh, 282004, India |
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| Abstract: | PurposeEvaluation of GeneXpert in comparison to liquid culture using Mycobacteria Growth Indicator Tube (MGIT) as gold standard for detection of Mycobacterium tuberculosis (MTB) in children.MethodsA total of 8123 samples, both pulmonary (5830) and extra pulmonary (2293) received from pediatric patients were processed for Acid Fast Bacilli (AFB) smear, GeneXpert and MGIT culture simultaneously.ResultsOut of 8123 samples, 493 (6.1%) samples were found positive by GeneXpert and 508 (6.2%) samples by MGIT culture, 371 (4.6%) were found positive by both GeneXpert and MGIT culture. MGIT detected 137 (1.7%) extra positive than GeneXpert while GeneXpert detected 122 (1.5%) extra samples more positive than by MGIT. Sensitivity of GeneXpert was 73% and concordance between both methods was 96.8%. Rifampicin resistance was found in 49 (9.9%) samples among MTB positive by GeneXpert. Turnaround time for GeneXpert was approx. 2 ?h and for MGIT, it was 12–28 days.ConclusionGood sensitivity (73%) and concordance (96.8%) were observed for GeneXpert against MGIT culture in this study. GeneXpert can simultaneously detect MTB and rifampicin resistance in less than 2 ?h while MGIT takes 12–28 days for MTB detection only. |
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| Keywords: | Extra pulmonary TB MGIT Pulmonary TB Rifampicin resistance |
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