艾塞那肽对乳腺癌MCF-7细胞增殖的影响

目的:研究艾塞那肽对人乳腺癌MCF-7细胞增殖的影响及涉及的信号通路。方法:艾塞那肽以不同浓度、时间作用于人乳腺癌MCF-7细胞,MTT法测细胞数目,流式细胞仪测定细胞周期,Western blot测细胞外信号调节蛋白激酶1/2(extracellular signal-regulated kinase1/2,Erk1/2)和蛋白激酶B(protein kinase B,Akt)磷酸化程度。结果:艾塞那肽1、10 nmol/L作用24、48 h或100、1 000 nmol/L作用24、48、72、96 h,MCF-7细胞数目减少,但差异无统计学意义,艾塞那肽1、10 nmol/L作用72、96 h,MCF-7细胞数目较对照组明显减少(P<0.05)。艾塞那肽10 nmol/L作用96 h,S+G2/M期细胞数目减少(P<0.05),同时出现Erk1/2磷酸化程度下降、Akt磷酸化程度升高,但差异无统计学意义。结论:艾塞那肽1、10 nmol/L作用MCF-7细胞72、96 h,抑制细胞增殖,该作用未涉及Erk和PI3K/Akt信号通路。

Effects of the exenatide on the proliferation of breast cancer cell MCF-7

Objective To investigate the the effects of the exenatide on the proliferation of breast cancer cell MCF-7 and the cell signaling pathways involved.Methods MCF-7 was treated with different concentrations of exenatide at different times.Cell number was determined by MTT assay,cell cycle was measured by flow cytometry and the phosphorylation of the Akt and Erk1/2 was evaluated by Western blot.Results MTT assay showed exenatide inhibit cell proliferation of MCF-7 significantly at the concentration of 1 and 10 nmol/L after being treated for 72 h and 96 h(P < 0.05),but no statistical significance of proliferation was found when MCF-7 was treated at the concentration of 100 and 1 000 nmol/L for 24 h to 96 h or at the the concentration 1 and 10 nmol/L for 24 h and 48 h(P > 0.05).When treated with exenatide for 96 h at 10 nmol/L,there was an increase of S+G2/M phase cell(P < 0.05),a decrease phosphorylation of Erk1/2 and an increase phosphorylation of Akt.However,no statistical significance of phosphorylation variations was found(P > 0.05).Conclusions Exenatide inhibit MCF-7cell proliferation at the concentration of 1 and 10 nmol/L after being treated for 72 h and 96 h,Erk/MAPK and PI3K/Akt pathway are not involved in this process.