子宫内膜异位症患者腹腔液T淋巴细胞亚群的改变及临床意义

目的:探讨子宫内膜异位症患者腹腔液T淋巴细胞亚群的改变及临床意义。方法:用流式细胞术检测子宫内膜异位症和子宫肌瘤患者的腹腔液T淋巴细胞亚群。结果:内异症患者腹腔液CD3+、CD4+T细胞及CD4+/CD8+比值均明显低于对照组,差异有显著性(P<0.01),内异症腹腔液CD8+T细胞与对照组相比无显著性差异(P>0.05)。内异症组腹腔液CD3+、CD4+、CD8+T细胞及CD4+/CD8+比值与子宫肌瘤组相比差异均无显著性(P>0.05)。与对照组相比,子宫肌瘤组腹腔液CD4+T细胞差异无显著性(P>0.05),而CD3+T细胞和CD4+/CD8+比值明显降低(P<0.01),CD8+T细胞明显升高(P<0.05)。轻度和重度子宫内膜异位症患者腹腔液CD3+、CD4+T细胞及CD4+/CD8+比值低于对照组,而CD8+T细胞高于对照组,差异均有显著性(P<0.05)。轻度和重度子宫内膜异位症相比,两组腹腔液CD3+、CD4+和CD8+T细胞差异均无显著性(P>0.05),重度组腹腔液CD4+/CD8+比值低于轻度组,差异有显著性(P<0.05)。结论:子宫内膜异位症的发生、发展与患者腹腔液的细胞免疫功能低下有关,检测腹腔液淋巴细胞亚群的变化对探讨其发病机理、预防和治疗均有重要的临床价值。

The variation of T lymphocyte subsets in peritoneal fluid of woman with endometriosis and its significance

Objective To investigate the variation of T lymphocyte subsets in peritoneal fluid of woman with endometriosis and its significance. Methods Flow cytometry was employed to demonstrate the difference in the number and ratio of the lymphocyte subsets between the patients with endometriosis and the controls. Results CD3+,CD4+ T cells and CD4+/CD8+ ratio in peritoneal fluid of woman with endometriosis were significantly lower than that of the controls (P < 0.01). No significant difference existed between the endometriosis group and the control group (P > 0.05). CD3+,CD4+,CD8+ T cells and CD4+/CD8+ ratio in peritoneal fluid of endometriosis woman were not significantly different with that of the uterine fibroids group (P > 0.05). There was no significant difference in peritoneal fluid CD4+ T cells between the uterine fibroids group and the control group (P > 0.05). The uterine fibroid woman contained lower CD3+ T cells and CD4+/CD8+ ratio (P < 0.01), and contained higher CD8+ T cells (P < 0.05) as compared with the control group. CD3+,CD4+ T cells and CD4+/CD8+ ratio in peritoneal fluid of woman with mild and severe endometriosis were significantly lower and CD8+ T cells significantly higher than that of the controls (P < 0.05). There was no significant difference in peritoneal fluid CD3+,CD4+ and CD8+ T cells between mild and severe endometriosis patients (P > 0.05). CD4+/CD8+ ratio in peritoneal fluid of the mild endometriosis was significantly lower than that in the severe endometriosis group (P < 0.05). Conclusions Cellular immune dysfunction in woman peritoneal fluid was related with pathogenesis of endometriosis. Test of lymphocyte subsets in woman peritoneal fluid had clinical importance in investigating the pathogenesis, prevention and treatment of endometriosis.