| 透明质酸对人骨关节炎软骨细胞保护机制的探讨 |
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| 引用本文： | 李化光,刘华,杨新明,张林西.透明质酸对人骨关节炎软骨细胞保护机制的探讨[J].实用医学杂志,2012,28(7):1040-1042. |
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| 作者姓名： | 李化光 刘华 杨新明 张林西 |
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| 作者单位： | 1. 河北北方学院附属第一医院骨科,河北省张家口市,075000
2. 河北北方学院生命科学研究中心,河北省张家口市,075000 |
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| 基金项目： | 张家口市科技局科学研究与发展指导计划项目 |
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| 摘 要： | 目的:探讨透明质酸对人骨关节炎软骨细胞的作用机制。方法:应用细胞培养、四唑盐比色实验检测细胞线粒体活性,流式细胞术检测线粒体膜电位、细胞凋亡百分率和细胞内游离钙离子浓度。结果:透明质酸能明显提高人骨关节炎软骨细胞线粒体活性,抬高线粒体膜电位,降低细胞凋亡率和胞内游离钙浓度。结论:透明质酸可抑制人骨关节炎软骨细胞线粒体膜电位的降低,从而具有稳定线粒体膜电位的作用,抑制细胞凋亡的发生,这种作用可能与其能抑制软骨细胞内钙超载有关。
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| 关 键 词： | 透明质酸 软骨细胞 钙 线粒体膜电位 |
Protective effects and mechanism of hyaluronic acid on human osteoarthritis chondrocytes |
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| LI Hua-guang
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LIU Hua
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YANG Xin-ming
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ZHANG Lin-xin.Protective effects and mechanism of hyaluronic acid on human osteoarthritis chondrocytes[J].The Journal of Practical Medicine,2012,28(7):1040-1042. |
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| Authors: | LI Hua-guang LIU Hua YANG Xin-ming ZHANG Lin-xin |
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| Institution: | n.* Department of Orthopaedics,The First Affiliated Hospital of Hebei North University,Zhangjiakou,07500,China |
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| Abstract: | Objective To study the effects and mechanism of hyaluronic acid on human osteoarthritis chondrocytes.Methods Mitochondrial activity was measured by methylthiazolyl tetrazolium test.Mitochondrial membrane protein(MMP),apoptosis and i were measured by flow cytometry.Results Hyaluronic acid significantly increased MMP and mitochondrial activity,and decreased i and apoptosis rate in human osteoarthritis chondrocytes.Conclusions Hyaluronic acid might inhibit the declines of MMP and mitochondrial activity,and has an inhibitory effect on osteoarthritis chondrocytes apoptosis.The mechanism might be related to inhibiting intracellular calcium overload. |
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| Keywords: | Hyaluronic acid Chondrocytes Calcium Mitochondrial membrane potential |
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