| 乙酰肼对大鼠肝微粒体细胞色素P450及亚型的作用 |
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| 引用本文： | 朱大岭 孟宪清. 乙酰肼对大鼠肝微粒体细胞色素P450及亚型的作用[J]. 哈尔滨医科大学学报, 1994, 28(5): 399-404 |
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| 作者姓名： | 朱大岭 孟宪清 |
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| 作者单位： | Zhu Daling;Meng Xianqing; Li Yulan;Gao Ying; Fang Deyuan;Song Chunfang;Xu Ping;Xu Jun(Metabolic Disease Laboratory，Harbin Medical University)(Control Center of Endemic Disease of China)(First Attached Hospital of Harbin Medical University) |
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| 摘 要： |
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| 关 键 词： | 乙酰肼 细胞色素 P450 亚型 单加氧酶 肝微粒体 |
Effect of Acetylhydrazine on the Hepatic Microsomal Cytochrome P450 and Its Isozyme in Rats |
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| Zhu Daling,Meng Xianqing, Li Yulan,Gao Ying, Fang Deyuan,Song Chunfang,Xu Ping,Xu Jun. Effect of Acetylhydrazine on the Hepatic Microsomal Cytochrome P450 and Its Isozyme in Rats[J]. Journal of Harbin Medical University, 1994, 28(5): 399-404 |
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| Authors: | Zhu Daling Meng Xianqing Li Yulan Gao Ying Fang Deyuan Song Chunfang Xu Ping Xu Jun |
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| Abstract: | The influence of acetylhydrazine(AHZ) on the hepatic microsomal cytochrome P450 and serum biochemistry were investigated in male rats. Changes in specific cytochrome P450 protein were monitored by using diagnostic substrates and immunologically polyclonal antibody.The activity of aniline hydroxylase,when expressed in terms of nmol. mg-1 microsomal protein, was increased by the treatment with AHZ.In contrast, the activity of benzphetamine N-demethylase significantly decreased.Immunoblot analysis showed that P450 2E was increased in AHZ treated rats, while the total microsomal cytochrome P450 was decreased.The serum level of GOT, GPT was also lower than that of control. These findings ihdicated that AHZ selectively induced microsomal cytochrome P450 2E while it repressed other microsomal P450 isozymes. |
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| Keywords: | Acetylhydrazine Cytochrome P450 P450 2E Monooxygenase activities |
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