毛叶香茶菜二萜成分的细胞毒活性研究

目的研究毛叶香茶菜的二萜成分及其细胞毒活性。方法利用硅胶柱层析分离和纯化毛叶香茶菜的二萜成分,用波谱分析方法鉴定其结构,用MTT法筛选其细胞毒活性。结果从毛叶香茶菜叶中分离鉴定14个已知对映-贝壳杉烷类二萜:effusainA(1),lasiodonin(2),skikokianin(3),rabdosichuaninD(4),oridion(5),lasiokaurin(6),1-αO--βD-glucopyranosyl-enmenol(7),rabdoterninA(8),epinodosin(9),epinodosinol(10),isodonal(11),isodonoiol(12),rabdosinB(13),rabdosinate(14);化合物1、2、3、5、6、9、11、12、13对人早幼粒白血病细胞(HL-60)、人卵巢癌细胞(HO-8910)和人肺癌细胞(A-549)的生长表现出强的抑制作用。结论毛叶香茶菜富含具有细胞毒活性的对映-贝壳杉烷类二萜。

Studies on cytotoxicity of the diterpenoids from Isodon japonica

Objective To research the diterpenoids of Isodon japonica and their cytotoxicity. Methods Diterpenoids of Isodon japonica were separated and purified by silica gel column chromatography. Their structures and cytotoxicity were determined by spectroscopic methods and MTT colorimetric assay, respectively.Results Fourteen known ent-kaurane diterpenoids were isolated and identified from the leaves of Isodon japonica: effusain A (1), lasiodonin (2), skikokianin (3), rabdosichuanin D (4), oridion (5), lasiokaurin (6), 1α-O-β-D-glucopyranosyl-enmenol (7), rabdoternin A (8), epinodosin (9), epinodosinol (10), isodonal (11), isodonoiol (12), rabdosin B (13), rabdosinate (14). Compounds 1, 2, 3, 5, 6, 9, 11, 12 and 13 exhibited significant inhibition on HL-60, HO-8910 and A-549 tumor cell lines. Conclusion Isodon japonica is abundant in Ent -kaurane diterpenoids with cytotoxicity.