| 制备高效液相色谱从竹根中制备苜蓿素的研究 |
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| 引用本文： | 张 锐,刘建群,刘丽艳,徐 彭. 制备高效液相色谱从竹根中制备苜蓿素的研究[J]. 医学教育探索, 2010, 41(12): 1985-1987 |
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| 作者姓名： | 张 锐 刘建群 刘丽艳 徐 彭 |
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| 作者单位： | 1.江西中医学院，江西 南昌330004； 2. 江西省肿瘤医院，江西 南昌330000 |
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| 基金项目： | 国家自然科学基金资助项目(30660220) |
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| 摘 要： | 目的 研究从竹根中制备苜蓿素对照品的方法。方法 结合硅胶柱色谱、制备HPLC法对竹根醋酸乙酯部位进行分离纯化，通过熔点及UV、IR波谱学方法对其分离产物进行结构鉴定。结果 所得苜蓿素用归一化法定量，质量分数大于98%。结论 本法简便，制备成本低，所得苜蓿素可作为中药定性、定量分析使用的对照品。
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| 关 键 词： | 竹根；苜蓿素；对照品；制备 |
Preparation of tricin from Bambusa textilis by preparative HPLC |
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| ZHANG Rui,LIU Jian-qun,LIU Li-yan,XUE Peng. Preparation of tricin from Bambusa textilis by preparative HPLC[J]. Researches in Medical Education, 2010, 41(12): 1985-1987 |
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| Authors: | ZHANG Rui LIU Jian-qun LIU Li-yan XUE Peng |
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| Affiliation: | 1. Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China; 2. Jiangxi Tumor Hospital, Nanchang 330000, China |
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| Abstract: | Objective To establish a separation method of tricin reference substance from Bambusa textilis. Methods After extracted with ethyl acetate, the extract of B. textilis was isolated and purified by silica gel column chromatography and preparative HPLC. Tricins were identified by melting point, UV, and IR spectroscopy. Results The content of tricin was over 98% by normalization method of HPLC. Conclusion The developed method is simple with lower cost, by which tricin can be used as reference substances for the qualitative and quantitative analyses of Chinese herbal medicine. |
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| Keywords: | Bambusa textilis Mc Clure tricin reference substance preparation |
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