| Significance and identification of p53 response element binding sequence in the genome of hepatitis B virus |
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| 引用本文： | 朱明华,段凌浔,戴益民,詹熔洲. Significance and identification of p53 response element binding sequence in the genome of hepatitis B virus[J]. 中国人民解放军军医大学学报, 1998, 0(4) |
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| 作者姓名： | 朱明华 段凌浔 戴益民 詹熔洲 |
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| 作者单位： | Department of Pathology，Fourth Military Medical University，Xi'an 710032，Shaanxi Province，ChinaDepartment of Pathology，Second Military Medical University，Shanghai 2 |
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| 摘 要： | ThereismuchevidencetosupportthatchronicinfectionofhepatitisBvirus(HBV)isoneoftheimportantfactorsrelatedwithprimaryhepatocellularcarcinoma(PHC)[IJ.ThemechanismbywhichHBVparticipatesinthedevelopmentofPHCremainstobeelucidatedyet.WereportedinpreviousstudiesthatP53proteincanbindHBxAgtoformthecomplexesofprotein--proteinbyimmunoprecipitationandincorporationinvitroandleadtop53inactivated[2-3j.WiththefindingthattheHBxproteinofHBVispresentinaproteincomplexcontainingp53,therearenowfivedifferent…
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Significance and identification of p53 response element binding sequence in the genome of hepatitis B virus |
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| Zhu Minghua,Duan Lingxun,Mark A. Feitelson,Roger J. Pomerantz,Dai Yimin,Zhan Rongzhou,. Significance and identification of p53 response element binding sequence in the genome of hepatitis B virus[J]. Journal of Medical Colleges of PLA(China), 1998, 0(4) |
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| Authors: | Zhu Minghua Duan Lingxun Mark A. Feitelson Roger J. Pomerantz Dai Yimin Zhan Rongzhou |
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| Abstract: | To explore the mechanism of interaction of hepatitis B virus (HBV) with P53 protein and its role in the hepatocarcinogenesis. Methods: HBV genome was analysed by computer program. The probe containing specific DNA-protein binding site in HBV genome was synthesized and reacted with nuclei protein of hepatoma cell lines. Specific binding was determined by electrophoretic mobility shift assay, electrophoretic mobility supershift assay and in situ ultraviolet cross-linking assay. Co-transfection was performed by using reporter gene cat, p53 and dexamethasone inducible HBx to observe the biological functions of HBV binding to p53 protein. Results: A p53 response element binding sequence is present in HBV genome at upstream of enhancer I from 1047 to 1059 hp. This sequence is capable of binding to p53 protein and increasing accumulation of p53 protein in cells. Conclusion: The results strongly suggest that DNA-protein binding of HBV with P53 protein plays a significant role and it may be the predominant mechanism in the pathogenesis of HBV-associated hepatocellular carcinoma. |
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| Keywords: | hepatitis B virus p53 response element tumor suppressor gene hepatoma |
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