| 标记死亡受体5、诱捕受体2地高辛探针 |
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| 引用本文： | 李家栋,王晓云,刘松,陈乾美. 标记死亡受体5、诱捕受体2地高辛探针[J]. 贵州医药, 2006, 30(8): 692-694 |
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| 作者姓名： | 李家栋 王晓云 刘松 陈乾美 |
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| 作者单位： | 贵阳医学院附属医院耳鼻咽喉科,550004;贵阳市第二人民医院脑科,550005 |
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| 摘 要： | 目的标记死亡受体5(death receptor-5,DR5/TRAILR2)和诱捕受体2(death decoyreceptor 2,DcR2/TRAILR4)地高辛探针。方法查阅核酸序列数据库上DR5和DcR2的基因全序列,计算机辅助设计引物,RT-PCR扩增目的条带,用地高辛(Digoxin,DIG)进行标记,制作DR5和DcR2地高辛探针。结果地高辛标记的DR5和DcR2电泳能力下降,标记成功。结论死亡受体5,诱捕受体2地高辛探针的标记成功为进一步研究其与喉鳞状细胞癌的关系奠定了一定的基础。
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| 关 键 词： | 死亡受体 诱捕受体 RT-PCR 地高辛 探针 |
| 文章编号： | 1000-744X（2006）08-0692-03 |
Label of the digoxin probe of the death receptor 5 and death decoy receptor 2 |
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| Li Jiadong, Wang Xiaoyun, Liu Song. Label of the digoxin probe of the death receptor 5 and death decoy receptor 2[J]. Guizhou Medical Journal, 2006, 30(8): 692-694 |
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| Authors: | Li Jiadong Wang Xiaoyun Liu Song |
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| Affiliation: | Department of otolaryngology, affiliated hospital of Guiyang Medical College, Guiyang 550004 |
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| Abstract: | Objective To label the digoxin probe of the death receptor 5(DR5) and death decoy receptor 2(DcR2).MethodsConsulting gene bankTM and analyzing conservative sequence of death receptor 5 and death decoy receptor 2,selecting a sequence as the primer and producing the target DNA by RT-PCR,then using DIG label.Results The electrophoretic mobility of labeled sample demonstrates a reduction in compared to an unlabeled control sample.Conclusion The successful label of the digoxin probe in the DR5 and DcR2 is very important to study the squamocellular carcinoma of larynx. |
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| Keywords: | Death receptor Death decoy receptor RT-PCR Digoxin Probe |
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