rhG-CSF动员健康供者CD34^＋细胞产率的影响因素分析

为探讨rhG—CSF动员健康供者年龄、性别等因素对采集物CD34^＋细胞产率的影响，分析61例健康供者外周血采集物CD34^＋细胞数量与自身特点的相关性，并进行多因素回归分析。以供者性别、年龄、身高、体重、体重指数（BMI）和采集时间作为研究参数，外周血采集物单核细胞计数、CD34^＋细胞占有核细胞百分比、CD34^＋细胞计数、CD34^＋细胞每公斤体重（供者）计数的均值作为研究变量。结果表明，供者年龄是影响CD34^＋细胞产率主要因素，呈中等负相关（-0．60〈r〈-0．45，P〈0．005）。偏相关分析排除身高、体重、BMI的影响，年龄仍和CD34^＋细胞产率呈中等负相关（-0．50〈r〈-0．35，P〈0．02）。BMI仅与CD34^＋细胞每公斤体重计数呈微弱负相关（r=-0．297，P〈0．05），性别对CD34^＋细胞产率无明显影响，CD34^＋细胞计数的差别仅出现在男性和女性低龄组（年龄〈35岁）间，男性身高、体重、BMI为CD34^＋细胞计数增加的有利因素。供者采集时间为给药后第4天采集，70％供者CD34^＋细胞产率达峰值。结论：年龄应作为供者选择的首要因素，性别、身高、体重和BMI对CD34^＋细胞产率的影响是次要的。

Factors Impacting Yield of CD34+ Cells from Healthy Donors Mobilized with rhG-CSF

This study was aimed to explore the effect of donor characteristics （ age, sex and so on. ） on CD34 ^＋ cell yields in apheresis from healthy donors mobilized by recombinant granulocyte colony-stimulating factor （rhG-CSF）. In 61 healthy donors, the characteristics associated with CD34^＋ cell yield were analysed. The relationship between the CD34 ^＋ cell yields and donor characteristics was statistically assessed with multivariate forward, backward and stepwise regression methods. A variety of parameters were analyzed which included donor age, sex, weight,height, body mass index （BMI） and time for collection of peripheral blood apheresis, while the mean number of peripheral blood mononuclear cells （MNCs）, CD34 ^＋ cell count, CD34^＋ cell proportion based on MNC and CD34 ＋ cell count per kg of donor weight were used as the variables. The results showed that age of donors was the main factor impacting CD34 ^＋ cell yields （ - 0.60 〈 r 〈 - 0.45, p 〈 0.005 ）. In a partial correlation analysis the body weight, height and BMI were served as control factors, the negative correlation of age with CD34 ^＋ cell yields was still found （ - 0.50 〈 r 〈 - 0.35, p 〈 0. 02 ）. BMI was only weakly correlated with the yields of CD34 ＋ cells per kg（ r = - 0.297, p 〈 0.05 ）. As a whole, sex showed no relation with the CD34^＋ cell yields. Compared with the female group less than 35 years old, height, weight and BMI in male group of low age exerted a positive impact on CD34^＋ cell yield. The optimal time for collection of PB was day 4 after treatment with rhG-CSF, when 70% of the donors could reach the peak CD34^＋ cell yields. It is concluded that the age of the donors is the first factor determing the choice of donors for allogeneic hematopoietic stem cell transplantation, the sex, height, weight and BMI are secondary factors impacting yield of CD34 ^＋cells from donors mobilized with rhG-CSF.