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HPLC测定红花中羟基红花黄色素A的含量
引用本文:雷志丹,雷志钧,夏新华,薛冰. HPLC测定红花中羟基红花黄色素A的含量[J]. 中国当代医药, 2011, 18(7): 45-46
作者姓名:雷志丹  雷志钧  夏新华  薛冰
作者单位:湖南中医药大学药学院,湖南长沙,410208
基金项目:湖南省教育厅重点课题,湖南省科技厅科学研究项目
摘    要:目的:建立HPLC法同时测定红花中羟基红花黄色素A的含量。方法:采用Kromasil C18(200mm×4.6mm,5μm)色谱柱,流动相为乙腈∶0.1%H3PO4=10∶90,流速1.0ml/min,检测波长403nm,柱温为30℃。结果:羟基红花黄色素A在0.89~10.68μg/ml的范围内呈良好的线性关系(r=0.9990)。结论:本测定方法快捷,简便,准确,重现性好。

关 键 词:高效液相色谱法  羟基红花黄色素A(HSYA)  红花

Determination of the content of hydroxysafior yellow A in Carthamus tinc-thus by HPLC
LEI Zhidan,LEI Zhijun,XIA Xinhua,XUE Bing. Determination of the content of hydroxysafior yellow A in Carthamus tinc-thus by HPLC[J]. http://www.botanicus.org/, 2011, 18(7): 45-46
Authors:LEI Zhidan  LEI Zhijun  XIA Xinhua  XUE Bing
Affiliation:(Pharmaceutical College,Hunan University of Traditional Chinese Medicine,Hunan Province,Changsha 410208,China)
Abstract:Objective: To develop an HPLC method for determining the content of hydroxysafflor yellow A Carthamus tinctrius.Methods: The analysis was performed on an Kromasil C18 (200 mm×4.6 mm,5 μm) column.The mobile phase consisted of acetonitrile: 0.1% phosphoric acid (10∶90).The flow rate was 1.0 ml/min.The detection wavelength was 403 nm and column temperature was 30℃.Results: The calibration curve was linear (r=0.999 0) in the range of 0.89-10.68 μg/ml for hydroxysafflor yellow A.Conclusion: The method is quick and simple,accurate and reproducible.
Keywords:HPLC  Hydroxysaff1or yellow A (HSYA)  Carthamus tinctrius L
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