龈沟液OPG、IL-35、IL-37在慢性牙周炎中的表达及与牙槽骨吸收的相关性

目的 探讨龈沟液骨保护素（osteoprotegerin，OPG）、白细胞介素35（interleukin-35，IL-35）、白细胞介素37（interleukin-37，IL-37）在慢性牙周炎中的表达及与牙槽骨吸收的相关性。 方法 选取慢性牙周炎患者105例作为试验组，慢性牙周炎患者45例作为验证组，健康体检者105例为对照组。比较试验组和对照组龈沟液OPG、IL-35、IL-37水平，比较试验组不同临床特征患者牙槽骨吸收比例情况，分析牙槽骨吸收比例的影响因素，建立多元线性回归模型。 结果 试验组龈沟液OPG、IL-37水平低于对照组，IL-35水平高于对照组（P＜0.05）。多元线性回归分析结果显示，龈沟液OPG、IL-35、IL-37水平是牙槽骨吸收比例的影响因素（P＜0.05）；建立回归模型为：牙槽骨吸收比例=6.495-0.180×OPG+0.169×IL-35-0.055×IL-37，自变量可解释牙槽骨吸收比例91.5%的变异量；采用验证组患者龈沟液OPG、IL-35、IL-37水平对回归模型进行验证，其平均绝对偏差值为1.25%。 结论 慢性牙周炎患者龈沟液OPG、IL-37低表达，IL-35高表达，三者均与牙槽骨吸收密切相关。

The expression of OPG,IL-35 and IL-37 in gingival crevicular fluid in chronic periodontitis and their correlation with alveolar bone resorption

ObjectiveTo explore the expression of osteoprotegerin(OPG), interleukin-35(IL-35) and interleukin-37(IL-37) in chronic periodontitis and their correlation with alveolar bone resorption.MethodsIn total, 105 patients with chronic periodontitis were selected as the experimental group, 45 patients with chronic periodontitis were selected as the verification group, and 105 patients undergoing physical examination were selected as the control group. The levels of OPG, IL-35 and IL-37 in the gingival crevicular fluid of the experimental group and the control group were compared. The proportion of alveolar bone resorption in patients with different clinical characteristics in the experimental group was compared. The influencing factors of resorption ratio of the alveolar bone in the experimental group were analyzed. A multiple linear regression model was established.ResultsThe levels of OPG and IL-37 in gingival crevicular fluid of the experimental group were lower than those of the control group, while the level of IL-35 was higher than that of the control group(P＜0.05). The results of multivariate linear regression analysis showed that the levels of OPG, IL-35 and IL-37 in gingival crevicular fluid were the influencing factors of resorption ratio of the alveolar bone(P＜0.05). The regression model was established as follows: Resorption ratio of the alveolar bone=6.495-0.180×OPG+0.169×IL-35-0.055×IL-37. The independent variable could explain the 91.5% variation of resorption ratio of the alveolar bone. The regression model was validated with the OPG, IL-35, and IL-37 levels in the patients′ gingival crevicular fluid in the verification group, and the mean absolute deviation(MAD) value was 1.25%.ConclusionThe expression of OPG and IL-37 in the gingival crevicular fluid of patients with chronic periodontitis is low, and the expression of IL-35 is high. The three are closely related to alveolar bone resorption.