miR-188-5p对胰岛素抵抗Hep G2细胞的改善作用研究

目的 探讨miR-188-5p对Hep G2细胞胰岛素抵抗的影响。 方法 采用高浓度葡萄糖（30 mmol/L）诱导处理人肝癌Hep G2细胞24 h构建胰岛素抵抗细胞模型，实验分为6组：对照组、模型组、miR-188-5p mimic组、mimic-NC组、miR-188-5p inhibitor组及inhibitor-NC组。实时荧光定量PCR（quantitative real-time PCR,qPCR)检测Hep G2细胞中miR-188-5p表达水平；MTT法检测细胞增殖率；生化实验检测细胞内葡萄糖及糖原含量；免疫荧光染色观察细胞中葡萄糖转运蛋白4（glucose transporter type 4 protein，GLUT4）的表达。 结果 与对照组比较，模型组miR-188-5p表达、细胞增殖率、葡萄糖摄取及糖原含量均明显降低（P＜0.05），GLUT4蛋白表达减少。与模型组比较，miR-188-5p mimic组miR-188-5p表达、细胞增殖率、葡萄糖摄取及糖原含量均明显升高（P＜0.05），GLUT4蛋白表达也增加，而miR-188-5p inhibitor组趋势与miR-188-5p mimic组相反。 结论 胰岛素抵抗Hep G2细胞中miR-188-5p表达降低，上调miR-188-5p表达可促进细胞增殖，缓解葡萄糖代谢障碍。

The effect of miR-188-5p on the improvement of insulin-resistant Hep G2 cells

Objective To investigate the effect of miR-188-5p on insulin resistance of Hep G2 cells.Methods Hep G2 cells were induced and treated with high concentration of glucose(30 mmol/L) for 24 h, to establish insulin resistance model. The experiment was divided into 6 groups: control group, model group, miR-188-5p mimic group, mimic-NC group, miR-188-5p inhibitor group, and inhibitor-NC group. The expression of miR-188-5p in Hep G2 cells was detected by qPCR, and the proliferation rate of Hep G2 cells was measured by MTT assay. The contents of glucose and glycogen in Hep G2 cells were detected by biochemical experiments, and the expression of glucose transporter type 4(GLUT4) was observed by immunofluorescence staining.Results Compared with the control group, miR-188-5p expression, cell proliferation rate, glucose uptake and glycogen content in the model group were significantly reduced(P＜0.05), and GLUT4 protein expression was reduced. Compared with the model group, the expression of miR-188-5p, cell proliferation rate, glucose uptake and glycogen content of miR-188-5p mimic group were significantly increased(P＜0.05), and the expression of GLUT4 protein was also increased, as opposed to miR-188-5p inhibitor group.Conclusion The expression of miR-188-5p is decreased in insulin-resistant Hep G2 cells. Up-regulation of miR-188-5p expression can promote cell proliferation and alleviate glucose metabolism disorders.