Significance of apoptosis related proteins on malignant transformation of ovarian tumors: A comparison between Bcl-2/Bax ratio and p53 immunoreactivity |
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| Institution: | 1. Department of Biophysics, Medical School, Celal Bayar University, Manisa, Turkey;2. Department of Histology and Embryology, Medical School, Celal Bayar University, Manisa, Turkey;3. Department of Pathology, Aegean Gynecology and Maternity Training and Research Hospital, Yenisehir, Izmir, Turkey;1. Department of Pharmacognosy and Phytochemistry, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Katowice, Poland;2. Silesian Medical College in Katowice, Katowice, Poland;1. Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, China;2. Laboratory of Translational Medicine, Jiangsu Provincial Academy of Traditional Chinese Medicine, Nanjing 210028, China;1. Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China;2. Research Center for Translational Medicine, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China;3. Department of Histology and Embryology, Shantou University Medical College, 22 Xin Ling Road, Shantou, Guangdong Province 515041, PR China;4. Burns Institute, The First Affiliated Hospital, Chinese PLA General Hospital, Trauma Center of Postgraduate Medical School, 51 Fu Cheng Road, Beijing 100037, PR China;1. Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada;2. Departments of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, CT 06520-8063, USA;3. Neurobiology, Yale University School of Medicine, New Haven, CT 06520-8063, USA;1. Division of Anatomy, Faculty of Dental Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania;2. MEDCENTER, Center of Excellence in Laboratory Medicine and Pathology, Bucharest, Romania;3. International Society of Regenerative Medicine and Surgery (ISRMS), Romania;4. Department of Medicine and Surgery, University of Catania, Italy;5. Division of Ophthalmology, S.C.U.O., Faculty of Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania;6. Clinical Hospital of Ophthalmologic Emergencies (S.C.U.O.), Bucharest, Romania |
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| Abstract: | In this study, we compared the immunoreactivities of Bcl-2, Bax and p53 proteins in ovarian tumors and related the immunohistochemical findings to the histological type of the tumors. Formalin-fixed, paraffin wax-embedded tissue sections from 40 patients who had serous-mucinous borderline tumors and serous-mucinous adenocarcinoma of the ovary (n = 10 each) were stained with hematoxylin–eosin (H&E). After histopathological examination, serial sections were stained immunohistochemically with primary antibodies to Bcl-2, Bax and p53 using an avidin–biotin-peroxidase method. A semi-quantitative grading system was used to compare the immunohistochemical staining intensities. The nuclear DNA fragmentation of apoptosis was determined using TUNEL method. As a result of immunohistochemical staining, increased immunoreactivity of Bcl-2 was observed in adenocarcinomas when compared to borderline tumors (P < 0.001). Strong immunoreactivity of Bcl-2 and mild immunoreactivities of Bax and p53 were detected in ovarian adenocarcinomas. There were no significant statistical differences in the immunoreactivity of Bax among the histological type of ovarian tumors. Whereas a balance was observed between the immunoreactivities of Bcl-2 and Bax in the borderline cases, and this balance was strongly changed toward the anti-apoptotic Bcl-2 protein in patients with adenocarcinoma. TUNEL staining of sections indicated apoptotic cells in the serous borderline tumors were about 8-fold higher than in the serous adenocarcinoma. The results of this study on apoptosis-related factors might help to develop novel protective and therapeutic approaches, such as isoflavonoids and isothiocyanates, which were associated with decreased Bcl-2/Bax ratio, against the malignant epithelial ovarian tumors. |
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| Keywords: | Apoptosis Bcl-2 protein family Immunoreactivity Ovarian tumor p53 |
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