育龄期女性MTHFR基因多态性观察及其与VitB12、Hcy、SFA的关系

目的研究育龄期女性5,10-亚甲基四氢叶酸还原酶(5,10-methylene tetrahydrofolate reductase, MTHFR)基因多态性及其与维生素B12(vitamin B12,VitB12)、同型半胱氨酸(homocysteine, Hcy)、叶酸(serum folic acid, SFA)的关系。方法选取既往有不良孕产史的围孕期患者124例设为A组;另选取行孕期检查且既往无不良孕产史的124例围孕期产妇设为B组。2组入院后均检测MTHFR、甲硫氨酸合成酶还原酶(methionine synthase reductase, MTRR)基因及血清VitB12、SFA、Hcy水平。比较2组MTHFR(C677T、A1298C)、MTRR(A66G)基因型及等位基因分布差异;比较2组血清VitB12、Hcy、SFA水平;比较不同MTHFR C677T基因型患者血清VitB12、Hcy、SFA水平。分析血清VitB12、Hcy、SFA水平与MTHFR C677T基因型间的相关性。结果 A组MTHFR C677T基因型CT、TT型及T等位基因分布检出率显著高于B组,差...

Observation of MTHFR gene polymorphism and its relationship with VitB12, Hcy and SFA in women of childbearing age

ObjectiveTo explore 5, 10-methylene tetrahydrofolate reductase(MTHFR) gene polymorphism and its relationship with vitamin B12(VitB12), homocysteine(Hcy) and folic acid(SFA) in women of childbearing age.MethodsIn total, 124 cases of perinatal patients with adverse pregnancy history were included as group A, and another 124 perinatal women who underwent pregnancy examination in our hospital during the same period and had no history of adverse pregnancy were included as group B. After admission, 5, 10-MTHFR, methionine synthase reductase(MTRR) genes and serum VitB12, SFA and Hcy were detected in both groups. MTHFR(C677T, A1298C), MTRR(A66G) genotype and allele distribution were compared between two groups. Serum VitB12, Hcy and SFA levels were compared between two groups. Serum VitB12, Hcy and SFA levels of patients with different MTHFR C677T genotypes were compared. In addition, the correlation between serum VitB12, Hcy, SFA levels and MTHFR C677T genotype was analyzed.ResultsThe detection rate of MTHFR C677T genotype CT, TT and T allele distribution in group A was significantly higher than that in group B, with statistically significant differences(P＜0.05). There was no significant difference in genotype and allele distribution of MTHFR A1298C between two groups(P＞0.05). No significant difference was found in MTHFR A66G genotype and allele distribution between two groups(P＞0.05). The Hcy level of group A was significantly higher than that of group B, and the SFA level was significantly lower than that of group B, with statistically significant differences(P＜0.05). The Hcy levels of MTHFR C677T genotype CT and TT were higher than those of CC genotype, and the SFA level was significantly lower than that of CC genotype, with statistically significant differences(P＜0.05). Correlation analysis confirmed that serum Hcy level was positively correlated with MTHFR C677T genotype CT and TT type, and serum SFA level was negatively correlated with MTHFR C677T genotype CT and TT type(P＜0.05).ConclusionThere are SFA and Hcy fluctuations and MTHFR C677T gene mutations in women of childbearing age with previous adverse pregnancy history. Timely supplementation of SFA for WOMEN of childbearing age with MTHFR C677T genotype CT and TT may reduce the incidence of adverse pregnancy and improve pregnancy outcome.