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Effect of low estrogen on neurons in the preoptic area of hypothalamus of ovariectomized rats |
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| Institution: | 1. Anatomy and Embryology Department, Peking University Health Science Center, No. 38 Xueyuan Road, Haidian District, Beijing, China;2. Obstetrics and Gynecology Department, Peking University First Hospital, No. 8 Xishiku Street, Xicheng District, Beijing, China;3. Statistics Office, Peking University First Hospital, No. 8 Xishiku Street, Xicheng District, Beijing, China;4. Department of Stomatology, General Hospital of Armed Police, No. 69 Yongding Road, Haidian District, Beijing, China;1. Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), U.Porto – University of Porto, Rua dos Bragas 289, P 4050-123 Porto, Portugal;2. Institute of Biomedical Sciences Abel Salazar (ICBAS), U.Porto – University of Porto, Laboratory of Histology and Embryology, Department of Microscopy, Rua Jorge Viterbo Ferreira 228, P 4050-313 Porto, Portugal;3. Faculty of Sciences (FCUP), U.Porto – University of Porto, Department of Biology, Rua do Campo Alegre, P 4169-007 Porto, Portugal;1. Division of Anatomy, Faculty of Dental Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania;2. MEDCENTER, Center of Excellence in Laboratory Medicine and Pathology, Bucharest, Romania;3. International Society of Regenerative Medicine and Surgery (ISRMS), Romania;4. Department of Medicine and Surgery, University of Catania, Italy;5. Division of Ophthalmology, S.C.U.O., Faculty of Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania;6. Clinical Hospital of Ophthalmologic Emergencies (S.C.U.O.), Bucharest, Romania;2. Department of Comparative Anatomy, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland;3. Poison Information Centre, Jagiellonian University Medical College, ?niadeckich 10, 31-531 Krakow, Poland;1. Faculdade de Medicina de São José do Rio Preto (FAMERP), Laboratório de Investigação Molecular do Câncer (LIMC), Sao Jose do Rio Preto, Sao Paulo 15090-000, Brazil;2. Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP/IBILCE), Program of Post-Graduate in Genetics, Sao Jose do Rio Preto, Sao Paulo 15054-000, Brazil;1. Department of Anatomy, Faculty of Medicine, P.J. ?afárik University, ?robárova 2, 041 80 Ko?ice, Slovak Republic;2. Department of Pharmacology, Faculty of Medicine, P.J. ?afárik University, tr. SNP 1, 040 11 Ko?ice, Slovak Republic |
| Abstract: | The purpose of this study was to investigate the difference in neuronal activity in the preoptic area of the hypothalamus (POAH) under low estrogen condition induced by ovariectomy. One hundred and twenty sham-operated (SHAM) and ovariectomized (OVX) rats were placed in different temperatures for 2 h. Twelve rats from each group were stimulated by 4 °C, 10 °C, 25 °C, 33 °C and 38 °C, respectively. c-Fos expression in the POAH was detected by immunohistochemistry. Following exposure to warm and cold stimuli, there were markedly lower c-Fos-positive cell densities in the OVX group compared with the SHAM group in the median preoptic nucleus (MnPO) at 4 °C, 10 °C, 33 °C and 38 °C, in the medial preoptic area (MPA) at 25 °C and 38 °C, in the ventromedial preoptic nucleus (VMPO) at 4 °C, 10 °C and 38 °C and in the ventrolateral preoptic nucleus (VLPO) at 4 °C and 38 °C. Both temperature and surgery had an impact on c-Fos expression by two-way ANOVA method except in the lateral preoptic area (LPO). c-Fos expression differed within different nuclei of the two groups in the same and different temperature stimuli. This indicated that the temperature-sensitive nuclei in the POAH exhibited lower and different activities during temperature stimuli following ovariectomy, which possibly resulted in abnormal thermoregulation and menopausal symptoms. |
| Keywords: | Preoptic area of hypothalamus (POAH) Temperature stimuli c-Fos Ovariectomized rats AH"} {"#name":"keyword" "$":{"id":"kw0030"} "$$":[{"#name":"text" "_":"hypothalamic area BLA"} {"#name":"keyword" "$":{"id":"kw0040"} "$$":[{"#name":"text" "_":"basolateral amygdaloid nuclei CeA"} {"#name":"keyword" "$":{"id":"kw0050"} "$$":[{"#name":"text" "_":"central amygdaloid nucleus DMH"} {"#name":"keyword" "$":{"id":"kw0060"} "$$":[{"#name":"text" "_":"dorsomedial hypothalamic nucleus LPB"} {"#name":"keyword" "$":{"id":"kw0070"} "$$":[{"#name":"text" "_":"lateral parabrachial nucleus LPO"} {"#name":"keyword" "$":{"id":"kw0080"} "$$":[{"#name":"text" "_":"lateral preoptic area MnPO"} {"#name":"keyword" "$":{"id":"kw0090"} "$$":[{"#name":"text" "_":"median preoptic nucleus MPA"} {"#name":"keyword" "$":{"id":"kw0100"} "$$":[{"#name":"text" "_":"medial preoptic area OVX"} {"#name":"keyword" "$":{"id":"kw0110"} "$$":[{"#name":"text" "_":"ovariectomized P5"} {"#name":"keyword" "$":{"id":"kw0120"} "$$":[{"#name":"text" "_":"peritrigeminal nucleus POAH"} {"#name":"keyword" "$":{"id":"kw0130"} "$$":[{"#name":"text" "_":"preoptic area of the hypothalamus SHAM"} {"#name":"keyword" "$":{"id":"kw0140"} "$$":[{"#name":"text" "_":"sham-operated VMPO"} {"#name":"keyword" "$":{"id":"kw0150"} "$$":[{"#name":"text" "_":"ventromedial preoptic nucleus VLPO"} {"#name":"keyword" "$":{"id":"kw0160"} "$$":[{"#name":"text" "_":"ventrolateral preoptic nucleus |
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