Mitochondrial DNA in the aquatic fungus Allomyces |
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| Authors: | Bernhard Borkhardt Mogens B Pedersen Lauritz W Olson |
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| Institution: | (1) Institute of Genetics, University of Copenhagen, Øster Farimagsgade 2A, DK-1353 Copenhagen K, Denmark;(2) Department of Brewing Chemistry, Carlsberg Research Laboratory, Gamle Carlsberg Vej 10, DK-2500 Copenhagen Valby, Denmark;(3) Present address: Institute of Plant Pathology, Lottenborgvej 2, DK-2800 Lyngby, Denmark;(4) Present address: Institute for Sporeplanter, University of Copenhagen, Øster Farimagsgade 2A, DK-1353 Copenhagen K, Denmark |
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| Abstract: | Summary The mitochondrial DNA from seven species of the aquatic phycomycete Allomyces has been isolated and characterized by restriction enzyme analysis. Comparison of the mitochondrial DNA restriction enzyme fragmentation patterns showed pronounced differences not only among species but also among four isolates of A. arbuscula. The mitochondrial DNAs range in size from 39 kbp in A. neo-moniliformis to 56 kbp in A. macrogynus.A physical map of the mitochondrial DNA of Allomyces arbuscula strain Costa Rica 21 has been constructed. The genome is circular and has a size of 49.2 kbp. The genes coding for the small and large ribosomal RNAs, cytochrome oxidase subunits 1, 2, and 3, apocytochrome b, and ATPase subunits 6 and 9 were localized in the mitochondria) DNA by heterologous hybridization with specific mitochondria) gene probes from Saccaromyces cerevisiae and Neurospora crassa. Comparison of the gene map of the closely related species Blastocladiella emersonii with that of A. arbuscula indicates a similar gene order in the two organisms. |
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| Keywords: | Allomyces MtDNA diversity Restriction mapping Gene localization |
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