| 透明质酸产生菌菌种诱变选育及发酵工艺研究 |
| |
| 引用本文： | 阎家麒 贾定武. 透明质酸产生菌菌种诱变选育及发酵工艺研究[J]. 中国生化药物杂志, 1994, 15(1): 21-24 |
| |
| 作者姓名： | 阎家麒 贾定武 |
| |
| 作者单位： | 郑州牧业工程高等专科学校!郑州450045(阎家麒)，河南医科大学(贾定武)，新乡市高科生物化学工程有限公司(赵敏) |
| |
| 摘 要： | 采用透明质酸酶缺陷型链球菌发酵生产透明质酸.从牛鼻粘膜分离兽疫链球菌,经诱变获得透明质酸酶缺陷型变异株Y-921.Y-921在含葡萄糖、蛋白胨、酵母浸膏及无机盐的发酵液中,pH7.0,37℃下发酵42h,得透明质酸4.5g/L,其分子量为1.9×10~6,蛋白质含量0.2%,这种高分子量透明质酸适用于化妆品.
|
| 关 键 词： | 透明质酸 发酵 诱变 兽疫链球菌 |
Selection of Hyaluronic Acid Producing Strains and Fermentative Manufacture |
| |
| Yan Jiaqi,Jia Dingwu,Zhao MinZhengzhou College of Animal Husbandry Engineering. Selection of Hyaluronic Acid Producing Strains and Fermentative Manufacture[J]. Chinese Journal of Biochemical Pharmaceutics, 1994, 15(1): 21-24 |
| |
| Authors: | Yan Jiaqi Jia Dingwu Zhao MinZhengzhou College of Animal Husbandry Engineering |
| |
| Affiliation: | Zhengzhou 450045 |
| |
| Abstract: | Hyaluronic acid is manufactured by cultivating a hyaluronidase-deficient Streptococcus mutant. S. zooepidemicus isolated from cattle tunica mucosa nasi was mutagenized to obtain a hyaluronidase-dificient strain Y-921. Y-921 was cultured in a medium containing glucose, peptone, yeast-extract and salt at 37℃, pH7. 0 for 42 h to product 4. 5 g hyaluronic acid/L. Its mol-wt was 1.9×10 6 daltons and its protein content was 0. 2%. The high mol-wt hyaluronic acid suitable for used in cosmetics. |
| |
| Keywords: | Hyaluronic acid Fermentation Variation S.zooepidemicus |
| 本文献已被 CNKI 维普 等数据库收录！ |
